PLS3 Polyklonaler Antikörper

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• KD/KO Validated

PLS3 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 55216-1-AP

Synonyme

Plastin 3, plastin 3 (T isoform), PLS3, T plastin

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 55216-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 55216-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 55216-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55216-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55216-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55216-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus using 55216-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 55216-1-AP (PLS3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human oesophagus using 55216-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 55216-1-AP (PLS3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 55216-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55216-1-AP (PLS3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55216-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55216-1-AP (PLS3 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, COLO 320-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ösophagusgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Produktinformation

55216-1-AP bindet in WB, IHC, ELISA PLS3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: plastin 3 (T isoform)
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 63 kDa, 38 kDa
• GenBank-Zugangsnummer: NM_005032
• Gene symbol: PLS3
• Gene ID (NCBI): 5358
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLS3, also named as T-plastin and Plastin-3, is an actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. It plays an important role in leukocyte function. Both T- and L-plastin are implicated in several diseases, L-plastin is considered to be a valuable marker for cancer. (PMID: 15960882) This antibody is specific to PLS3

Publikationen

Species	Application	Title
human	WB,IHC	J Cell Physiol; PLS3 predicts poor prognosis in pancreatic cancer and promotes cancer cell proliferation via PI3K/AKT signaling.; Authors - Zechang Xin ; KD Validated
human	WB,IHC	Environ Toxicol; PLS3 promotes papillary thyroid carcinoma progression by activating the Notch signaling pathway; Authors - Dongtao Wang ; KD Validated
human	WB	J Cell Biochem; Phosphorylation of mitochondrial phospholipid scramblase 3 by protein kinase C-delta induces its activation and facilitates mitochondrial targeting of tBid.; Authors - Yongwen He ; KD Validated
mouse	WB	Biochim Biophys Acta; Comparative proteomic analysis of mouse models of pathological and physiological cardiac hypertrophy, with selection of biomarkers of pathological hypertrophy by integrative Proteogenomics.; Authors - Hye Kyeong Kwon