PLSCR1 Polyklonaler Antikörper

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• KD/KO Validated

PLSCR1 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-Fro, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-Fro, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Rezensionen (1)

Kat-Nr. : 11582-1-AP

Synonyme

Ca(2+)-dependent phospholipid scramblase 1, EC:3.1.-.-, Mg(2+)-dependent nuclease, MMTRA1B, phospholipid scramblase 1

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  WB analysis using 11582-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11582-1-AP

  WB result of PLSCR1 antibody (11582-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLSCR1 transfected HEK-293 cells.

• 

  WB analysis using 11582-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11582-1-AP

  Mouse lung lysates were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 11582-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11582-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 11582-1-AP

  IP result of anti-PLSCR1 (IP:11582-1-AP, 4ug; Detection:11582-1-AP 1:500) with mouse lung tissue lysate 2800ug.

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  IHC staining of mouse spleen using 11582-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11582-1-AP (PLSCR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11582-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11582-1-AP (PLSCR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 11582-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11582-1-AP (PLSCR1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11582-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11582-1-AP (PLSCR1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse spleen using 11582-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11582-1-AP (PLSCR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 11582-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using PLSCR1 antibody (11582-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of rat brain using 11582-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using PLSCR1 antibody (11582-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HeLa using 11582-1-AP

  Immunofluorescent analysis of HeLa cells using 11582-1-AP (PLSCR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, humanes Skelettmuskelgewebe, Mauslungengewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: Mausmilzgewebe, humanes Nierengewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-Fro: Rattenhirngewebe, Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 14 publications below
• IHC: See 5 publications below
• IF: See 8 publications below
• IP: See 2 publications below

Produktinformation

11582-1-AP bindet in WB, IHC, IF/ICC, IF-Fro, IP, ELISA PLSCR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLSCR1 fusion protein Ag2172
• Vollständiger Name: phospholipid scramblase 1
• Berechnetes Molekulargewicht: 318 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC021100
• Gene symbol: PLSCR1
• Gene ID (NCBI): 5359
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLSCR1 (Phospholipid scramblase 1) is a member of the family of membrane proteins that mediate the transbilayer movement of phospholipids ("scrambling") in a Ca2+ dependent manner, which was identified as an a type II transmembrane protein involved in the calcium-dependent, nonspecific, rapid redistribution of phospholipids. A cysteine-rich palmitoylation motif [184CCCPCC189] of PLSCR1, a thioester linkage to the sulfhydryl groups of cysteines, controls the transportation of PLSCR1 to the cell membrane or nucleus (PMID: 32292520). PLSCR1 is strongly expressed in response to interferon (IFN) treatment and viral infection (PMID: 35138119, 23487022).

Publikationen

Species	Application	Title
mouse	WB,IF	J Clin Invest; Phospholipid scramblase-1 regulates innate type 2 inflammation in mouse lung via CRTH2-dependent mechanisms; Authors - Ashley Hernandez-Gutierrez
human	WB	PLoS Pathog; Phospholipid scramblase 1 interacts with influenza A virus NP, impairing its nuclear import and thereby suppressing virus replication.; Authors - Weiyu Luo
mouse	WB	J Neurosci; Phospholipid Scramblase 1 Controls Efficient Neurotransmission and Synaptic Vesicle Retrieval at Cerebellar Synapses; Authors - Margherita Caputo ; KD Validated
human	WB,IF	J Proteome Res; An Interactome Map Reveals Phospholipid Scramblase 1 as a Novel Regulator of Hepatitis B Virus X Protein.; Authors - Yanzhi Yuan ; KD Validated
human	WB,IP,IF	Cancer Sci; Nuclear accumulation of KPNA2 impacts radioresistance through positive regulation of the PLSCR1-STAT1 loop in lung adenocarcinoma.; Authors - Wei-Chao Liao ; KD Validated
mouse	WB	J Leukoc Biol; Phospholipid scramblase 1 is required for β2-glycoprotein I binding in hypoxia and reoxygenation-induced endothelial inflammation.; Authors - Emily Archer Slone

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Teresa (Verified Customer) (01-31-2025)

The antibody gives a quite good signal in IF, however you need a strong secondary antibody for it. I still need to improve experimental setup conditions.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:30
• Cell Tissue Type: A549-ACE2, HEK293T