PMPCB Polyklonaler Antikörper

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• KD/KO Validated

PMPCB Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Kat-Nr. : 16064-1-AP

Synonyme

Beta MPP, MPP11, MPPB, MPPP52, P 52, PMPCB

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 16064-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16064-1-AP (PMPCB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16064-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16064-1-AP (PMPCB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16064-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16064-1-AP (PMPCB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 16064-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16064-1-AP (PMPCB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16064-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16064-1-AP (PMPCB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 16064-1-AP

  IP Result of anti-PMPCB (IP:16064-1-AP, 3ug; Detection:16064-1-AP 1:500) with MCF-7 cells lysate 2560ug.

• 

  IHC staining of human lung cancer using 16064-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16064-1-AP (PMPCB antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16064-1-AP

  Immunofluorescent analysis of HepG2 cells using 16064-1-AP (PMPCB antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 16064-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16064-1-AP (PMPCB antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, HepG2-Zellen, humanes Lebergewebe, MCF-7-Zellen, Rattenlebergewebe
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Produktinformation

16064-1-AP bindet in WB, IP, IHC, IF, ELISA PMPCB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PMPCB fusion protein Ag8937
• Vollständiger Name: peptidase (mitochondrial processing) beta
• Berechnetes Molekulargewicht: 489 aa, 54 kDa
• Beobachtetes Molekulargewicht: 43-52 kDa
• GenBank-Zugangsnummer: BC010398
• Gene symbol: PMPCB
• Gene ID (NCBI): 9512
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PMPCB(Mitochondrial-processing peptidase subunit beta) is also named as MPPB, P-52 and belongs to the peptidase M16 family. It cleaves presequences (transit peptides) from mitochondrial protein precursors. It is a mitochondrial matrix marker and Tom70, cytochrome c heme lyase (CCHL) are markers for outer membrane, intermembrane space(PMID:12574118).

Publikationen

Species	Application	Title
human	WB	Nature; Mitochondrial stress is relayed to the cytosol by an OMA1-DELE1-HRI pathway.; Authors - Xiaoyan Guo
human	WB	Nat Cell Biol; PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.; Authors - Shotaro Saita
human	WB	Nat Commun; DELE1 tracks perturbed protein import and processing in human mitochondria.; Authors - Evelyn Fessler
human	WB	EMBO J; PARL partitions the lipid transfer protein STARD7 between the cytosol and mitochondria.; Authors - Shotaro Saita
	WB,IF	Autophagy; The TOMM machinery is a molecular switch in PINK1 and PARK2/PARKIN-dependent mitochondrial clearance.; Authors - Giulia Bertolin ; KD Validated
human	WB	Cell Rep; Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.; Authors - Fenglei Jian