PMS1 Polyklonaler Antikörper

PMS1 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 28186-1-AP

Synonyme

DKFZp781M0253, FLJ98259, HNPCC3, hPMS1, PMS1, PMS1 protein homolog 1, PMSL1

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Galerie der Validierungsdaten

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  WB analysis using 28186-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28186-1-AP (PMS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28186-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 28186-1-AP (PMS1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IF Staining of Caco-2 using 28186-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PMS1 antibody (28186-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, MOLT-4-Zellen
• Erfolgreiche Detektion in IF: Caco-2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

28186-1-AP bindet in WB, IF, ELISA PMS1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PMS1 fusion protein Ag28132
• Vollständiger Name: PMS1 postmeiotic segregation increased 1 (S. cerevisiae)
• Beobachtetes Molekulargewicht: 106 kDa
• GenBank-Zugangsnummer: BC096331
• Gene symbol: PMS1
• Gene ID (NCBI): 5378
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PMS1 Homolog 1 is a member of the DNA mismatch repair enzymes (MMREs) which is to eliminate the mismatch of insertions and deletions as a consequence of DNA polymerase errors at DNA synthesis in eukaryotes. PMS1 and MLH1 could form a heterodimer MutLβ that belongs to MMREs (PMID:25619773). In the MMR system, the MutS complex recognizes the DNA mispairs firstly, and then the Mlh1-Pms1 and other associated proteins such as PCNA, RFC, ExoI were recruited, inducing Exonuclease 1 (Exo1)-dependent and -independent MMR pathways (PMID:24981171). Mutations in this gene cause hereditary nonpolyposis colorectal cancer(HNPCC), which is also known as Lynch syndrome. It was reported that PMS1 is a widely expressed protein and located in the nucleus. Alternative splicing allows for 4 isoforms of PMS1 protein.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daniela (Verified Customer) (03-17-2022)

The staining was aspecific and very weak.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Gastric mouse organoids

PMS1 Antibody Immunofluorescence validation (1:100 dilution) in Gastric mouse organoids (Cat no:28186-1-AP)