PNPLA6 Polyklonaler Antikörper

PNPLA6 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 14261-1-AP

Synonyme

Neuropathy target esterase, NTE, NTEMND, PNPLA6, SPG39, sws

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 14261-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14261-1-AP (PNPLA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 14261-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14261-1-AP (PNPLA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment using 14261-1-AP

  IP result of anti-PNPLA6 (14261-1-AP for IP and Detection) with HEK-293 cells.

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  IF Staining of HepG2 using 14261-1-AP

  Immunofluorescent analysis of HepG2 cells, using PNPLA6 antibody 14261-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of MCF-7 using 14261-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PNPLA6 antibody 14261-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, SH-SY5Y-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

14261-1-AP bindet in WB, IP, IF, ELISA PNPLA6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PNPLA6 fusion protein Ag5546
• Vollständiger Name: patatin-like phospholipase domain containing 6
• Berechnetes Molekulargewicht: 150 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC051768
• Gene symbol: PNPLA6
• Gene ID (NCBI): 10908
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PNPLA6(patatin-like phospholipase domain-containing protein 6) is also named as NTE(neuropathy target esterase) and belongs to the NTE family. It plays an important role in motor neuron development and implicated overexpression of BMP signaling(PMID:22996643). Defects in PNPLA6 are the cause of spastic paraplegia autosomal recessive type 39 (SPG39)(PMID:18313024). This protein has a glycosylaion site that it can be glycosylated(PMID:9576844). It has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Nature; FMRP targets distinct mRNA sequence elements to regulate protein expression.; Authors - Ascano Manuel M Jr
		Mol Biol Cell; Lowe syndrome-linked endocytic adaptors direct membrane cycling kinetics with OCRL in Dictyostelium discoideum.; Authors - Alexandre Luscher