POLD3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

POLD3 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 21935-1-AP

Synonyme

DNA polymerase delta subunit 3, KIAA0039, P66, P68, POLD3

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  WB analysis using 21935-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21935-1-AP (POLD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 21935-1-AP

  IP result of anti-POLD3 (IP:21935-1-AP, 4ug; Detection:21935-1-AP 1:500) with MCF-7 cells lysate 640 ug.

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  IHC staining of human breast cancer using 21935-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21935-1-AP (POLD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 21935-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21935-1-AP (POLD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 21935-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21935-1-AP (POLD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21935-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21935-1-AP (POLD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below

Produktinformation

21935-1-AP bindet in WB, IHC, IP, ELISA POLD3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POLD3 fusion protein Ag16570
• Vollständiger Name: polymerase (DNA-directed), delta 3, accessory subunit
• Berechnetes Molekulargewicht: 466 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51-70 kDa
• GenBank-Zugangsnummer: BC108908
• Gene symbol: POLD3
• Gene ID (NCBI): 10714
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Genes Dev; Break-induced replication promotes fragile telomere formation.; Authors - Zhe Yang ; KO Validated
mouse	WB	Genes Dev; TRF1 uses a noncanonical function of TFIIH to promote telomere replication; Authors - Zhe Yang ; KD Validated
human	WB	J Adv Res; Bavachinin Protects the Liver in NAFLD by Promoting Regeneration via Targeting PCNA; Authors - Xi Dong
human		Mol Cell; Mechanisms of insertions at a DNA double-strand break; Authors - Jaewon Min
mouse	WB	Nucleic Acids Res; Somatic and intergenerational G4C2 hexanucleotide repeat instability in a human C9orf72 knock-in mouse model; Authors - Nada Kojak
mouse	WB	Nucleic Acids Res; Disruption of G-quadruplex dynamicity by BRCA2 abrogation instigates phase separation and break-induced replication at telomeres; Authors - Jennifer J Lee