SKAR Polyklonaler Antikörper

SKAR Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 17466-1-AP

Synonyme

POLDIP3, KIAA1649, p46, PDIP3, PDIP46

---

• 

  WB analysis of HeLa using 17466-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 17466-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17466-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17466-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17466-1-AP

  IP result of anti-SKAR (IP:17466-1-AP, 4ug; Detection:17466-1-AP 1:300) with mouse brain tissue lysate 4000 ug.

• 

  IHC staining of human stomach using 17466-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17466-1-AP (SKAR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 17466-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17466-1-AP (SKAR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17466-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKAR antibody (17466-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HepG2-Zellen, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

17466-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SKAR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SKAR fusion protein Ag11338
• Vollständiger Name: polymerase (DNA-directed), delta interacting protein 3
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC019643
• Gene symbol: SKAR
• Gene ID (NCBI): 84271
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Oncotarget; PDIP46 (DNA polymerase δ interacting protein 46) is an activating factor for human DNA polymerase δ.; Authors - Xiaoxiao Wang
human	WB,IF	Sci Rep; TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.; Authors - S Prpar Mihevc
human	WB	Am J Cancer Res; Inhibition of mTORC1 signaling sensitizes hepatocellular carcinoma cells to glycolytic stress.; Authors - Xin Zhao