POLR1C Polyklonaler Antikörper

POLR1C Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 15923-1-AP

Synonyme

AC40, POLR1C, POLR1E, RPA39, RPA40, RPA5, RPAC1, RPC40

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  WB analysis using 15923-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15923-1-AP (POLR1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15923-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15923-1-AP (POLR1C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 15923-1-AP

  IP result of anti-POLR1C (IP:15923-1-AP, 4ug; Detection:15923-1-AP 1:800) with NIH/3T3 cells lysate 1600 ug.

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  IHC staining of human ovary using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse liver using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15923-1-AP (POLR1C antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15923-1-AP (POLR1C antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human placenta using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human spleen using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, Caco-2-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Eierstockgewebe, humanes Herzgewebe, humanes Nierengewebe, humanes Lebergewebe, humanes Plazenta-Gewebe, humanes Hautgewebe, humanes Milzgewebe, humanes Hodengewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

15923-1-AP bindet in WB, IHC, IP, ELISA POLR1C und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POLR1C fusion protein Ag8746
• Vollständiger Name: polymerase (RNA) I polypeptide C, 30kDa
• Berechnetes Molekulargewicht: 346 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC008863
• Gene symbol: POLR1C
• Gene ID (NCBI): 9533
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

POLR1C is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is a common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).

Publikationen

Species	Application	Title
mouse		Genome Biol; Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture; Authors - Yongpeng Jiang
human	WB	Biochim Biophys Acta Mol Cell Res; POLR1D, a shared subunit of RNA polymerase I and III, modulates mTORC1 activity; Authors - Neuton Gorjão

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Krishnendu (Verified Customer) (02-27-2020)

Nice antibody. Got a specific band.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T