POLR2B-Specific Polyklonaler Antikörper

POLR2B-Specific Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20370-1-AP

Synonyme

hRPB140, hsRPB2, POL2RB, POLR2B, POLR2B-Specific, RNA polymerase II subunit 2, RNA polymerase II subunit B2, RPB2

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Galerie der Validierungsdaten

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  WB analysis of A431 using 20370-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20370-1-AP (POLR2B-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20370-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20370-1-AP (POLR2B-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20370-1-AP

  IP Result of anti-POLR2B-Specific (IP:20370-1-AP, 4ug; Detection:20370-1-AP 1:300) with HeLa cells lysate 3000ug.

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  IP experiment of HeLa using 20370-1-AP

  IP Result of anti-POLR2B-Specific (IP:20370-1-AP, 4ug; Detection:20370-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of human brain using 20370-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20370-1-AP (POLR2B-Specific antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human brain using 20370-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20370-1-AP (POLR2B-Specific antibody) at dilution of 1:400 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

20370-1-AP bindet in WB, IP, IHC, ChIP, ELISA POLR2B-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
• Berechnetes Molekulargewicht: 134 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: NM_000938
• Gene symbol: POLR2B
• Gene ID (NCBI): 5431
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

POLR2B belongs to the RNA polymerase beta chain family. It is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is the second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. POLR2B proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. The antibody is specific to POLR2B.

Publikationen

Species	Application	Title
human	ChIP	Biochem Pharmacol; MCP-1 facilitates VEGF production by removing miR-374b-5p blocking of VEGF mRNA translation; Authors - Huan-Yu Zhao
human	WB	Cancer Invest; Quinacrine Depletes BCR-ABL and Suppresses Ph-Positive Leukemia Cells; Authors - Hu Lei