POP1 Polyklonaler Antikörper

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• KD/KO Validated

POP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Rezensionen (1)

Kat-Nr. : 12029-1-AP

Synonyme

POP 1

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• 

  WB analysis using 12029-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12029-1-AP (POP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12029-1-AP

  WB result of POP1 antibody (12029-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-POP1 transfected HeLa cells.

• 

  IP experiment of HeLa using 12029-1-AP

  IP result of anti-POP1 (IP:12029-1-AP, 4ug; Detection:12029-1-AP 1:8000) with HeLa cells lysate 1560 ug.

• 

  IHC staining of human ovary cancer using 12029-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12029-1-AP (POP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12029-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POP1 antibody (12029-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IP: See 2 publications below
• RIP: See 2 publications below

Produktinformation

12029-1-AP bindet in WB, IHC, IF/ICC, IP, RIP, ELISA POP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POP1 fusion protein Ag2660
• Vollständiger Name: processing of precursor 1, ribonuclease P/MRP subunit (S. cerevisiae)
• Berechnetes Molekulargewicht: 1024 aa, 115 kDa
• Beobachtetes Molekulargewicht: 115 kDa
• GenBank-Zugangsnummer: BC011529
• Gene symbol: POP1
• Gene ID (NCBI): 10940
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Processing of Precursor 1 (POP1), a core component of RNase subunits, is an autoantigen in patients with connective tissue disease and is involved in suppressing inflammation.

Publikationen

Species	Application	Title
human	WB	Mol Cell; HSP70 binds to specific non-coding RNA and regulates human RNA polymerase III; Authors - Sergio Leone
human	WB	Mol Cell; Endoribonucleolytic Cleavage of m6A-Containing RNAs by RNase P/MRP Complex.; Authors - Ok Hyun Park
human	WB	Nat Microbiol; The SARS-CoV-2 RNA-protein interactome in infected human cells.; Authors - Nora Schmidt
human	WB,RIP,IHC	Research (Wash D C); POP1 Facilitates Proliferation in Triple-Negative Breast Cancer via m6A-Dependent Degradation of CDKN1A mRNA; Authors - Chao Zhang
human	WB	Cell Rep; UPF1 promotes rapid degradation of m6A-containing RNAs.; Authors - Sung Ho Boo
human	WB,IP	Cell Rep; m1A and m6A modifications function cooperatively to facilitate rapid mRNA degradation; Authors - Sung Ho Boo ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Estefania (Verified Customer) (05-16-2022)

Many bands appearing. Protease inhibitor not used for the preparation of cell lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HepG2 cell lysate

POP1 Antibody Western Blot validation (1:500 dilution) in HepG2 cell lysate (Cat no:12029-1-AP)