POPDC3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

POPDC3 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 11800-1-AP

Synonyme

POP3, POPDC3, popeye domain containing 3, Popeye protein 3, RP11 99L11.2

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Galerie der Validierungsdaten

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  WB analysis using 11800-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11800-1-AP (POPDC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 11800-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11800-1-AP (POPDC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11800-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11800-1-AP (POPDC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11800-1-AP

  IP Result of anti-POPDC3 (IP:11800-1-AP, 3ug; Detection:11800-1-AP 1:1000) with HeLa cells lysate 220ug.

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  IHC staining of mouse heart using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11800-1-AP (POPDC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11800-1-AP (POPDC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 11800-1-AP (POPDC3 Antibody) at dilution of 1:50.

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  IHC staining of human placenta using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 11800-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 11800-1-AP (POPDC3 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HEK-293-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Plazenta-Gewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 3 publications below

Produktinformation

11800-1-AP bindet in WB, IP, IHC, ELISA POPDC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POPDC3 fusion protein Ag2375
• Vollständiger Name: popeye domain containing 3
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC022323
• Gene symbol: POPDC3
• Gene ID (NCBI): 64208
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Popeye domain-containing (POPDC) gene family includes BVES/POPDC1, POPDC2 and POPDC3. POPDC proteins are highly evolutionarily conserved transmembrane proteins expressed in embryonic epithelium, heart and muscle. POPDC3 is a 291-amino acid protein containing three putative transmembrane domains. It has been reported that POPDC3 expression is reduced in gastric cancer, suggesting it might play an important role in the progression and metastases of gastric cancer. (PMID: 10882522; 20627872; 22654436)

Publikationen

Species	Application	Title
human	WB,IHC	Carcinogenesis; Frequent silencing of popeye domain-containing genes, BVES and POPDC3, is associated with promoter hypermethylation in gastric cancer.; Authors - Kim Mirang M ; KD Validated
human	IHC	Front Oncol; Study on differentially expressed genes between stage M and stage MS neuroblastoma; Authors - Yuying Wu
human	IHC	World J Gastroenterol; Reduced Popdc3 expression correlates with high risk and poor survival in patients with gastric cancer.; Authors - Luo Deng D

Verwandte Produkte

BVES Polyclonal antibody

12920-1-AP