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PPCS Polyklonaler Antikörper

PPCS Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 18001-1-AP

Synonyme

COAB, PPC synthetase, PPCS

Filter:
  • Western Blot (WB) analysis of HeLa cells using PPCS Polyclonal antibody (18001-1-AP)

    WB analysis of HeLa using 18001-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18001-1-AP (PPCS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human kidney using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human kidney using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human placenta using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human placenta using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human testis using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human testis using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human spleen using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human spleen using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human lung using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human lung using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human ovary using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human ovary using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen
Erfolgreiche Detektion in IHChumanes Nierengewebe, humanes Lungengewebe, humanes Eierstockgewebe, humanes Plazenta-Gewebe, humanes Milzgewebe, humanes Hodengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunhistochemie (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IFSee 1 publications below

Produktinformation

18001-1-AP bindet in WB, IF, IHC, ELISA PPCS und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PPCS fusion protein Ag12491
Vollständiger Name phosphopantothenoylcysteine synthetase
Berechnetes Molekulargewicht 311 aa, 34 kDa
Beobachtetes Molekulargewicht 34 kDa
GenBank-ZugangsnummerBC106064
Gene symbol PPCS
Gene ID (NCBI) 79717
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPCS(Phosphopantothenate--cysteine ligase) is also named as COAB and belongs to the PPC synthetase family. It catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. It can be exsit as a homodimer(PMID:12906824).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for PPCS antibody 18001-1-APProtokoll herunterladen
IHC protocol for PPCS antibody 18001-1-APProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIF

Int J Mol Sci

Regulation of the CoA Biosynthetic Complex Assembly in Mammalian Cells.

Authors - Jovana Baković
View Article

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Jovana (Verified Customer) (09-17-2020)

The antibody worked well to detect endogenous PPCS protein on WB in HEK293 cells. Dilution used was 1:1000 and incubation was 2h at RT.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mammalian cells