CYPC Polyklonaler Antikörper

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• KD/KO Validated

CYPC Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 10287-2-AP

Synonyme

Cyclophilin C, CYPC, PPIase C, PPIC, Rotamase C

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 10287-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10287-2-AP (CYPC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10287-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10287-2-AP (CYPC antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 10287-2-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 10287-2-AP (CYPC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10287-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10287-2-AP (CYPC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 10287-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10287-2-AP (CYPC Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 10287-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10287-2-AP (CYPC Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 10287-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10287-2-AP (CYPC Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse kidney using 10287-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10287-2-AP (CYPC Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 10287-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYPC antibody (10287-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HEK-293 using 10287-2-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 10287-2-AP (CYPC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HuH-7-Zellen, Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below

Produktinformation

10287-2-AP bindet in WB, IHC, IF, ELISA CYPC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYPC fusion protein Ag0209
• Vollständiger Name: peptidylprolyl isomerase C (cyclophilin C)
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC002678
• Gene symbol: PPIC
• Gene ID (NCBI): 5480
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclophilin C also named as peptidyl-prolyl cis-trans isomerase C(PPIC), is expressed in several bone marrow (BM) hematopoietic progenitors and in T‐cell precursors(PMID: 33864384). CYPC/PPIC catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. Similar to other PPIases, this protein can bind immunosuppressant cyclosporin A. In addition, CYPC/PPIC is involved in many biological processes, including mitochondrial metabolism, REDOX and inflammation, as well as related diseases and conditions, such as ischemia-reperfusion injury, AIDS and cancer.

Publikationen

Species	Application	Title
human	WB	J Biol Chem; The isomerase active site of cyclophilin A is critical for hepatitis C virus replication.; Authors - Udayan Chatterji ; KD Validated
human	WB	J Biol Chem; Depletion of Cyclophilins B and C Leads to Dysregulation of Endoplasmic Reticulum Redox Homeostasis.; Authors - Pawel Stocki
human	WB	PLoS One; Cyclophilin C Participates in the US2-Mediated Degradation of Major Histocompatibility Complex Class I Molecules.; Authors - Daniel C Chapman
human	WB	Int J Mol Sci; Pharmacological Targeting of the ER-Resident Chaperones GRP94 or Cyclophilin B Induces Secretion of IL-22 Binding Protein Isoform-1 (IL-22BPi1).; Authors - Paloma Gómez-Fernández
human	WB	ACS Chem Neurosci; Furanoditerpenes from Spongia (Spongia) tubulifera Display Mitochondrial-Mediated Neuroprotective Effects by Targeting Cyclophilin D.; Authors - Rebeca Alvariño
mouse	WB	Front Physiol; Extracellular cyclophilins A and C induce dysfunction of pancreatic microendothelial cells; Authors - Rebeca Alvariño