Cyclophilin E Polyklonaler Antikörper

Cyclophilin E Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Rezensionen (3)

Kat-Nr. : 15357-1-AP

Synonyme

PPIE, Cyclophilin 33, Cyclophilin-33, CYP 33, CYP33

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  WB analysis using 15357-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15357-1-AP (Cyclophilin E antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15357-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15357-1-AP (Cyclophilin E antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 15357-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclophilin E antibody (15357-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15357-1-AP bindet in WB, IF/ICC, ELISA Cyclophilin E und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cyclophilin E fusion protein Ag7603
• Vollständiger Name: peptidylprolyl isomerase E (cyclophilin E)
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33-38 kDa
• GenBank-Zugangsnummer: BC004898
• Gene symbol: Cyclophilin E
• Gene ID (NCBI): 10450
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclophilin E (CypE), also known as Cyclophilin-33, Rotamase E, CYP33, PPIE, is an enzyme which belongs to the cyclophilin-type PPIase family of PPIase E subfamily (PMID: 15998457). Cyclophilin E negatively regulates influenza virus replication and transcription occurs by impairing the formation of the vRNP (PMID: 21887220). Additionally, Cyclophilin E plays an important role in osteoblast differentiation as a positive regulator by increasing the transcriptional activity of Runx2 (PMID: 37947627).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (07-19-2025)

Overall antibody recognize the band of the expected size, but there are several non-specific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: RKO

Cyclophilin E Antibody Western Blot validation (1:2000 dilution) in RKO (Cat no:15357-1-AP)

FH

Moritz (Verified Customer) (09-12-2024)

Several bands were observable in WB, also the expected size of PPIE, although it was not the strongest.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293T

Cyclophilin E Antibody Western Blot validation (1:3000 dilution) in HEK293T (Cat no:15357-1-AP)

FH

Tsimafei (Verified Customer) (08-12-2024)

Multiple bands are present, however the one corresponding to Cyclophilin E size (~33 kDa) is clearly visible

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mESCs

Cyclophilin E Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:15357-1-AP)