PPM1B Polyklonaler Antikörper

PPM1B Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, Cell treatment, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 13193-1-AP

Synonyme

PP2C beta, PP2C beta X, PP2CB, PP2CBETA, PPC2BETAX, PPM1B, Protein phosphatase 1B

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Galerie der Validierungsdaten

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  WB analysis using 13193-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13193-1-AP (PPM1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 13193-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13193-1-AP (PPM1B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 13193-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13193-1-AP (PPM1B Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skeletal muscle using 13193-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13193-1-AP (PPM1B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 13193-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13193-1-AP (PPM1B Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of MCF-7 using 13193-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 13193-1-AP (PPM1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below
• IP: See 2 publications below

Produktinformation

13193-1-AP bindet in WB, IP, IHC, IF, Cell treatment, ELISA PPM1B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPM1B fusion protein Ag3871
• Vollständiger Name: protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform
• Berechnetes Molekulargewicht: 479 aa, 53 kDa
• Beobachtetes Molekulargewicht: 36, 53 kDa
• GenBank-Zugangsnummer: BC012002
• Gene symbol: PPM1B
• Gene ID (NCBI): 5495
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPM1B is a member of the metal-dependent serine/threonine protein phosphatase (PPM) family with important regulatory functions in cellular signalling pathways(PMID: 18066953). PPM1B is also an important negative regulator of several stress signalling pathways and has been shown to negatively regulate the stress-activated p38 and JNK pathways by directly dephosphorylating upstream signalling proteins(PMID: 9824284). Moreover, PPM1B has also been shown to prevent cell death by negatively regulating necrotic cell death in a RIP3 dephosphorylation-dependent manner.(PMID: 25751141) PPM1B can be detected as the WM of 36-55 kDa.

Publikationen

Species	Application	Title
human	IP	Nat Commun; USP9X regulates centrosome duplication and promotes breast carcinogenesis.; Authors - Xin Li
mouse	IP,IF,Cell treatment	Cell Rep; Blocking AMPK β1 myristoylation enhances AMPK activity and protects mice from high-fat diet-induced obesity and hepatic steatosis; Authors - Katyayanee Neopane
human		Biochim Biophys Acta Mol Cell Res; TXLNA enhances TBK1 phosphorylation by suppressing PPM1B recruitment; Authors - Xiao Tian
mouse		Front Pharmacol; Initial Characterization of Stressed Transgenic Mice With Cardiomyocyte-Specific Overexpression of Protein Phosphatase 2C.; Authors - Paula Bollmann