PPM1F Polyklonaler Antikörper

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• KD/KO Validated

PPM1F Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 17020-1-AP

Synonyme

Ca(2+)/calmodulin-dependent protein kinase phosphatase, CaM kinase phosphatase, CaM-kinase phosphatase, CaMKPase, EC:3.1.3.16

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  WB analysis of MCF-7 using 17020-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17020-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17020-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 17020-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 17020-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 17020-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17020-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 17020-1-AP

  IP result of anti-PPM1F (IP:17020-1-AP, 4ug; Detection:17020-1-AP 1:700) with MCF-7 cells lysate 2800ug.

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  IHC staining of human colon cancer using 17020-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17020-1-AP (PPM1F antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 17020-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17020-1-AP (PPM1F antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 17020-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PPM1F antibody (17020-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Lebergewebe, K-562-Zellen, L02-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

17020-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PPM1F und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPM1F fusion protein Ag10186
• Vollständiger Name: protein phosphatase 1F (PP2C domain containing)
• Berechnetes Molekulargewicht: 453 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC071989
• Gene symbol: PPM1F
• Gene ID (NCBI): 9647
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mg2+/Mn2+-dependent protein phosphatase 1F (PPM1F) belongs to the PP2C family of Ser/Thr protein phosphatases and is the critical enzyme responsible for dephosphorylating the threonine motif. PPM1F is ubiquitous in various tissues and organs (PMID: 26498692). High expression of PPM1F was positively associated with poor survival and tumor recurrence in patients with cancers and PPM1F might be a potential biomarker in HCC patients (PMID: 30470261).

Publikationen

Species	Application	Title
mouse	WB	J Cell Biol; PPM1F controls integrin activity via a conserved phospho-switch.; Authors - Tanja M Grimm ; KO Validated
human	WB	Clin Transl Oncol; PPM1F regulates ovarian cancer progression by affecting the dephosphorylation of ITGB1; Authors - Yahui Leng