PDP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PDP1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 21176-1-AP

Synonyme

PPM2C, [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial, EC:3.1.3.43, PDP 1, PDPC 1

---

• 

  WB analysis of PC-3 using 21176-1-AP

  WB result of PDP1 antibody (21176-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDP1 transfected PC-3 cells.

• 

  WB analysis using 21176-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21176-1-AP (PPM2C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21176-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21176-1-AP (PPM2C Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 21176-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21176-1-AP (PPM2C Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 21176-1-AP

  IP result of anti-PDP1 (IP:21176-1-AP, 4ug; Detection:21176-1-AP 1:300) with mouse brain tissue lysate 5200ug.

• 

  IHC staining of human prostate cancer using 21176-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21176-1-AP (PPM2C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 21176-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21176-1-AP (PPM2C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 21176-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21176-1-AP (PPM2C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 21176-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21176-1-AP (PPM2C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, Maushirngewebe, Mausherzgewebe, Mauslebergewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Schilddrüsenkarzinomgewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below

Produktinformation

21176-1-AP bindet in WB, IHC, IP, ELISA PDP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDP1 fusion protein Ag15453
• Vollständiger Name: protein phosphatase 2C, magnesium-dependent, catalytic subunit
• Berechnetes Molekulargewicht: 537 aa, 61 kDa
• Beobachtetes Molekulargewicht: 55-61 kDa
• GenBank-Zugangsnummer: BC047619
• Gene symbol: PDP1
• Gene ID (NCBI): 54704
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pyruvate dehydrogenase phosphatase 1 (PDP1) is a key hormone-regulated metabolic enzyme that dephosphorylates and activates pyruvate dehydrogenase (PDH), thereby stimulating the conversion of pyruvate into acetyl-CoA (PMID: 36453802). PDP1 expression is regulated by FLT3-ITD in a context-dependent manner, and that it is centrally involved in the adaptation of AML glucose metabolism to the requirements of proliferation, survival and the therapeutic response to FLT3 inhibition (PMID: 37935978).

Publikationen

Species	Application	Title
human	IHC	Aging (Albany NY); Identification of immune-related cells and genes in the breast invasive carcinoma microenvironment.; Authors - Xingfeng Chen
human	WB	Curr Med Sci; Salidroside Inhibits Lipopolysaccharide-ethanol-induced Activation of Proinflammatory Macrophages via Notch Signaling Pathway.; Authors - Jian-Sha Li
mouse	WB	J Diabetes Res; Pyruvate-Enriched Oral Rehydration Solution Improves Glucometabolic Disorders in the Kidneys of Diabetic db/db Mice.; Authors - Xiao Meng Zhang
human	WB	Cell Death Dis; Mitochondrial dysfunction induces radioresistance in colorectal cancer by activating [Ca2+]m-PDP1-PDH-histone acetylation retrograde signaling.; Authors - Yingying Shi
mouse	WB	ACS Chem Neurosci; FDCA Attenuates Neuroinflammation and Brain Injury after Cerebral Ischemic Stroke; Authors - Xin Guan
mouse	WB	J Physiol; Monocarboxylate transporter 4 deficiency enhances high-intensity interval training-induced metabolic adaptations in skeletal muscle; Authors - Yuki Tamura