PPP1CA Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

PPP1CA Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte, Zebrafisch

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

1C10A2

Kat-Nr. : 67070-1-Ig

Synonyme

PP 1A, PPP1A, PPP1CA

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Galerie der Validierungsdaten

• 

  WB analysis using 67070-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67070-1-Ig (PPP1CA antibody) at dilution of 1:2600 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis of zebrafish using 67070-1-Ig

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 67070-1-Ig (PPP1CA antibody) at dilution of 1:2600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67070-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67070-1-Ig (PPP1CA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 67070-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67070-1-Ig (PPP1CA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67070-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67070-1-Ig (PPP1CA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, fetales humanes Hirngewebe, Hausschwein-Hirngewebe, HEK-293-Zellen, Jurkat-Zellen, Kaninchenhirngewebe, Maushirngewebe, Rattenhirngewebe, T-47D-Zellen, Zebrafischgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IP: See 1 publications below

Produktinformation

67070-1-Ig bindet in WB, IP, ELISA PPP1CA und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten, Zebrafisch

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte, Zebrafisch
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PPP1CA fusion protein Ag28451
• Vollständiger Name: protein phosphatase 1, catalytic subunit, alpha isoform
• Berechnetes Molekulargewicht: 330 aa, 38 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC001888
• Gene symbol: PPP1CA
• Gene ID (NCBI): 5499
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein phosphatase associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is essential for cell division and participates in the regulation of glycogen metabolism, muscle contractility, and protein synthesis. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (PMID: 9835651). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (PMID: 8384581, PMID: 1392080). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (PMID: 9122166).

Publikationen

Species	Application	Title
human	WB,IP	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang ; KD Validated
mouse	WB	J Neurosci; CREB inactivation by HDAC1/PP1γ contributes to dopaminergic neurodegeneration in Parkinson's disease.; Authors - Xiaoyi Xu 许潇依
rat	WB	Neurotoxicology; Nuclear accumulation of histone deacetylase 4 (HDAC4) by PP1-mediated dephosphorylation exerts neurotoxicity in Pb-exposed neural cells.; Authors - Xiaozhen Gu
human	WB	Exp Ther Med; Small interfering RNA-mediated knockdown of KRT80 suppresses colorectal cancer proliferation.; Authors - Jiatian Lin
human,mouse	WB	Zool Res; EPAS1 prevents telomeric damage-induced senescence by enhancing transcription of TRF1, TRF2, and RAD50; Authors - Kai-Qin Li ; KD Validated
human	WB	Acta Pharm Sin B; Predicting and overcoming resistance to CDK9 inhibitors for cancer therapy; Authors - Chen Hu