PPP1CB-Specific Polyklonaler Antikörper

PPP1CB-Specific Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10140-2-AP

Synonyme

PP1 Beta, PP 1B, PPP1CB, PPP1CD

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 10140-2-AP

  NIH3T3 cells were subjected to SDS PAGE followed by western blot with 10140-2-AP (PPP1CB-Specific antibody) at dilution of 1:1500.

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  WB analysis of human brain using 10140-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10140-2-AP (PPP1CB-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10140-2-AP

  IP Result of anti-PPP1CB-Specific (IP:10140-2-AP, 4ug; Detection:10140-2-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IP experiment of mouse brain using 10140-2-AP

  IP Result of anti-PPP1CB-Specific (IP:10140-2-AP, 4ug; Detection:10140-2-AP 1:500) with mouse brain tissue lysate 2640ug.

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  IHC staining of human prostate cancer using 10140-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10140-2-AP (PPP1CB-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of NIH/3T3 using 10140-2-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 10140-2-AP (PPP1CB-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, NIH/3T3
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

10140-2-AP bindet in WB, IP, IHC, IF,ELISA PPP1CB-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP1CB-Specific fusion protein Ag0183
• Vollständiger Name: protein phosphatase 1, catalytic subunit, beta isoform
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC002697
• Gene symbol: PPP1CB
• Gene ID (NCBI): 5500
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein phosphatase 1 (PP1) is the main eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular processes including protein-protein interactions, gene transcription, cell-cycle progression and apoptosis. It is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. The catalytic subunit of PP1 (PP1c) can interact with amount of regulatory subunits in vivo that target it to particular subcellular locations and regulate its activity towards specific substrates. PPP1CB migrated as a 37kd protein on SDS page. This antibody is specific to PPP1CB.

Publikationen

Species	Application	Title
human	WB	Am J Physiol Cell Physiol; PP1γ functionally augments the alternative splicing of CaMKIIδ through interacting with ASF.; Authors - Chao Huang
human	WB,IHC	Melanoma Res; Differential expression patterns of capping protein, protein phosphatase 1, and casein kinase 1 may serve as diagnostic markers for malignant melanoma.; Authors - Sun Daxin D
rat	WB, IP	Clin Exp Pharmacol Physiol; Rescue of cardiac failing and remodeling by inhibition of PP1γ is associated with suppression of the ASF-mediated splicing of CaMKIIδ; Authors - Ru-Jia Liao