PPP1CC Polyklonaler Antikörper

PPP1CC Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 11082-1-AP

Synonyme

PP1 Gamma, PP 1G, PPP1CC, PPP1G

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Galerie der Validierungsdaten

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  WB analysis using 11082-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11082-1-AP (PPP1CC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11082-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11082-1-AP (PPP1CC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11082-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11082-1-AP (PPP1CC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 11082-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 11082-1-AP (PPP1CC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11082-1-AP

  IP Result of anti-PPP1CC (IP:11082-1-AP, 4ug; Detection:11082-1-AP 1:500) with HEK-293 cells lysate 2000ug.

• 

  IHC staining of human pancreas cancer using 11082-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11082-1-AP (PPP1CC antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11082-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11082-1-AP (PPP1CC antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 11082-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 11082-1-AP (PPP1CC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HEK-293T-Zellen, L02-Zellen, Maushirngewebe, MCF-7-Zellen, Rattenhirngewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IP: See 2 publications below

Produktinformation

11082-1-AP bindet in WB, IP, IHC, IF, ELISA PPP1CC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP1CC fusion protein Ag1550
• Vollständiger Name: protein phosphatase 1, catalytic subunit, gamma isoform
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC014073
• Gene symbol: PPP1CC
• Gene ID (NCBI): 5501
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPP1CC, also named as PP-1G, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. It is involved in regulation of ionic conductances and long-term synaptic plasticity. PPP1CC may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II.

Publikationen

Species	Application	Title
human	WB	J Biol Chem; Dephosphorylation activates the interferon-stimulated schlafen family member 11 in the DNA Damage Response.; Authors - Dane Malone
human	WB	Am J Physiol Cell Physiol; PP1γ functionally augments the alternative splicing of CaMKIIδ through interacting with ASF.; Authors - Chao Huang
rat	WB, IP	Clin Exp Pharmacol Physiol; Rescue of cardiac failing and remodeling by inhibition of PP1γ is associated with suppression of the ASF-mediated splicing of CaMKIIδ; Authors - Ru-Jia Liao
human	WB	Cell Death Dis; Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration; Authors - Xiaoman Zhu
human,mouse	WB,IP	Dev Cell; Loss of RanGAP1 drives chromosome instability and rapid tumorigenesis of osteosarcoma; Authors - Yan Gong ; KD Validated
human,mouse	WB	Zool Res; EPAS1 prevents telomeric damage-induced senescence by enhancing transcription of TRF1, TRF2, and RAD50; Authors - Kai-Qin Li ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jua (Verified Customer) (08-01-2018)

• Applications: Flow Cytometry,
• Primary Antibody Dilution: Souris têtard isralien