PPP1CC Polyklonaler Antikörper

PPP1CC Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 55150-1-AP

Synonyme

PP1 Gamma, PP 1G, PP1C gamma, PPP1CC, PPP1G

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Galerie der Validierungsdaten

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  WB analysis using 55150-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55150-1-AP (PPP1CC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 55150-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 55150-1-AP (PPP1CC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55150-1-AP

  IP Result of anti-PPP1CC (IP:55150-1-AP, 4ug; Detection:55150-1-AP 1:500) with mouse brain tissue lysate 3440ug.

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  IHC staining of human heart using 55150-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55150-1-AP (PPP1CC antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 55150-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55150-1-AP (PPP1CC antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 55150-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55150-1-AP (PPP1CC antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 55150-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55150-1-AP (PPP1CC antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 55150-1-AP

  Immunofluorescent analysis of HeLa cells using 55150-1-AP (PPP1CC antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, L02-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

55150-1-AP bindet in WB, IP, IHC, IF PPP1CC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein phosphatase 1, catalytic subunit, gamma isoform
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: NM_002710
• Gene symbol: PPP1CC
• Gene ID (NCBI): 5501
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPP1CC, also named as PP-1G, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. It is involved in regulation of ionic conductances and long-term synaptic plasticity. PPP1CC may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. The antibody is specific to PPP1CC.

Publikationen

Species	Application	Title
mouse	WB	Front Cell Dev Biol; PERK Signaling Controls Myoblast Differentiation by Regulating MicroRNA Networks.; Authors - Ye-Ya Tan
mouse	WB	J Biol Chem; Hydrogen sulfide modulates eukaryotic translation initiation factor 2α (eIF2α) phosphorylation status in the integrated stress response pathway.; Authors - Vinita Yadav
human	WB	Cell Death Dis; Genome-wide identification of microRNA targets reveals positive regulation of the Hippo pathway by miR-122 during liver development.; Authors - Yin Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Vinita (Verified Customer) (12-04-2018)

• Applications: Western Blot,
• Cell Tissue Type: Hek293