PPP1R15B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PPP1R15B Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Rezensionen (2)

Kat-Nr. : 14634-1-AP

Synonyme

CREP, PPP1R15B

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Galerie der Validierungsdaten

• 

  WB analysis of SGC-7901 using 14634-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 14634-1-AP (PPP1R15B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14634-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14634-1-AP (PPP1R15B antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14634-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14634-1-AP (PPP1R15B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14634-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14634-1-AP (PPP1R15B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 14634-1-AP

  IP Result of anti-PPP1R15B;CReP (IP:14634-1-AP, 3ug; Detection:14634-1-AP 1:1000) with MCF-7 cells lysate 2500ug.

• 

  IHC staining of mouse brain using 14634-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14634-1-AP (PPP1R15B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 14634-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14634-1-AP (PPP1R15B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14634-1-AP

  Immunofluorescent analysis of HepG2 cells using 14634-1-AP (PPP1R15B antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IgG .

• 

  IF Staining of SH-SY5Y using 14634-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PPP1R15B antibody (14634-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, HEK-293-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen, SGC-7901-Zellen, SKOV-3-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 19 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

14634-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA PPP1R15B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP1R15B fusion protein Ag6213
• Vollständiger Name: protein phosphatase 1, regulatory (inhibitor) subunit 15B
• Berechnetes Molekulargewicht: 79 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC065280
• Gene symbol: PPP1R15B
• Gene ID (NCBI): 84919
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein phosphatase 1 regulatory subunit 15B(PPP1R15B) is a member of PPP1R15 family, it forms a complex with protein phosphatase1(PP1) and NCK1/2, maintaining low levels of EIF2S1 phosphorylation in unstressed cells, thus is also named constitutive repressor of eIF2alpha phosphorylation (CReP). PPP1R15B is assoicated in endoplasmic reticulum (ER) stress response resulting to a role in translation regulation. Knock-down of PPP1R15B strongly protected mammalian cells against oxidative stress, peroxynitrite stress, and more modestly against accumulation of malfolded proteins in the ER. Recently, PPP1R15B was found to associate with cell membranes and regulate membrane traffic in a PP1c-independent manner, suggesting a novel link between translation and traffic. MW of PPP1R15B is 80 kDa. Observed MW of PPP1R15B is from 100-110 kDa maybe due to phosphorylation (PMID: 28492545).

Publikationen

Species	Application	Title
human	WB	Cell; Target-Based Discovery of an Inhibitor of the Regulatory Phosphatase PPP1R15B.; Authors - Agnieszka Krzyzosiak
mouse	WB	Neuron; Excitatory neuron-specific suppression of the integrated stress response contributes to autism-related phenotypes in fragile X syndrome; Authors - Mehdi Hooshmandi
mouse	WB	Hepatology; PMID: 29698569; Authors - Xu Xu ; KO Validated
human	WB,IF	Sci Adv; CReP mediates selective translation initiation at the endoplasmic reticulum.; Authors - Jonathan P Kastan
mouse	WB	Nat Commun; Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis.; Authors - Hongtan Wu
mouse	WB	Proc Natl Acad Sci U S A; An inhibitor of HIV-1 protease modulates constitutive eIF2α dephosphorylation to trigger a specific integrated stress response.; Authors - Aude De Gassart

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (03-11-2023)

Detects a major band ~100 kDa and shows upregulation in stressed cells (MG132 treatment). WB: 4% milk in TBST and semi-dry transfer; detects a double band at ~55kDa. Also was tested in IHC in mouse CNS tissue and in ICC in human cells; the signal appears specific in both cases (not shown).

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human, SH-SY5Y cells; mouse, brain

PPP1R15B Antibody Western Blot,Immunohistochemistry,Immunofluorescence validation (1:1000 dilution) in Human, SH-SY5Y cells; mouse, brain (Cat no:14634-1-AP)

FH

Will (Verified Customer) (10-24-2019)

good antibody, recommended by a colleague, would order again

• Applications: Western Blot,
• Cell Tissue Type: hepatocytes

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