PPP1R3B Polyklonaler Antikörper

PPP1R3B Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27285-1-AP

Synonyme

PTG, PPP1R4, PP1 subunit R4, Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL

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  WB analysis of mouse heart using 27285-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 27285-1-AP (PPP1R3B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 27285-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 27285-1-AP (PPP1R3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 27285-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27285-1-AP (PPP1R3B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 27285-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27285-1-AP (PPP1R3B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, MDA-MB-453s-Zellen
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27285-1-AP bindet in WB, IHC, ELISA PPP1R3B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP1R3B fusion protein Ag26025
• Vollständiger Name: protein phosphatase 1, regulatory (inhibitor) subunit 3B
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC043388
• Gene symbol: PPP1R3B
• Gene ID (NCBI): 79660
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPP1R3B, also known as Protein Phosphatase 1, Regulatory Subunit 3B, is a protein encoded by the PPP1R3B gene in humans. It is a regulatory subunit of the serine/threonine-specific protein phosphatase 1 (PP1), an enzyme that plays a crucial role in the control of a wide range of cellular processes by dephosphorylating target proteins.