PPP1R8 Polyklonaler Antikörper

PPP1R8 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16115-1-AP

Synonyme

NIPP1, NIPP 1, ARD-1, ARD1, ARD 1

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  WB analysis of PC-3 using 16115-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16115-1-AP (PPP1R8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16115-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16115-1-AP (PPP1R8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 16115-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16115-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 16115-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16115-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16115-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16115-1-AP bindet in WB, IHC, IF/ICC, ELISA PPP1R8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP1R8 fusion protein Ag9041
• Vollständiger Name: protein phosphatase 1, regulatory (inhibitor) subunit 8
• Berechnetes Molekulargewicht: 209 aa, 23 kDa
• Beobachtetes Molekulargewicht: 47 kDa, 28 kDa, 19 kDa
• GenBank-Zugangsnummer: BC013360
• Gene symbol: PPP1R8
• Gene ID (NCBI): 5511
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPP1R8, also named as ARD1, NIPP1, is a 351 amino acid protein, which has a basic N- and C-terminal and an acidic central domain. PPP1R8 is ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. PPP1R8 has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates and may also be involved in pre-mRNA splicing. PPP1R8 exists many isoforms and molecular weight of modified isoforms is about 18 kDa, 28 kDa and 47 kDa.