PPP1R9A Polyklonaler Antikörper

PPP1R9A Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18801-1-AP

Synonyme

KIAA1222, Neurabin 1, Neurabin I, NRB1, NRBI, PPP1R9A

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Galerie der Validierungsdaten

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  WB analysis of PC-3 using 18801-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18801-1-AP (PPP1R9A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 18801-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18801-1-AP (PPP1R9A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 18801-1-AP

  Immunofluorescent analysis of HepG2 cells, using PPP1R9A antibody 18801-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 18801-1-AP

  Immunofluorescent analysis of Hela cells, using PPP1R9A antibody 18801-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18801-1-AP bindet in WB, IF,ELISA PPP1R9A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein phosphatase 1, regulatory (inhibitor) subunit 9A
• Berechnetes Molekulargewicht: 123 kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: BC150636
• Gene symbol: PPP1R9A
• Gene ID (NCBI): 55607
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPP1R9A, also named as KIAA1222 and NEB1, binds to actin filaments (F-actin) and shows cross-linking activity. PPP1R9A has been found to be concentrated in the synapse of developed neurons and in the lamellipodia of the growth cone during neuron development.PPP1R9A may be involved in neurite formation. PPP1R9A inhibits protein phosphatase 1-alpha activity.