PPP2R1A Polyklonaler Antikörper

PPP2R1A Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 15882-1-AP

Synonyme

PPP2R1A, PR65A

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Galerie der Validierungsdaten

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  WB analysis using 15882-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15882-1-AP (PPP2R1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15882-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15882-1-AP (PPP2R1A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 15882-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15882-1-AP (PPP2R1A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15882-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15882-1-AP (PPP2R1A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 15882-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 15882-1-AP (PPP2R1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15882-1-AP

  IP Result of anti-PPP2R1A (IP:15882-1-AP, 4ug; Detection:15882-1-AP 1:300) with HepG2 cells lysate 3200ug.

• 

  IHC staining of human kidney using 15882-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15882-1-AP (PPP2R1A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15882-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15882-1-AP (PPP2R1A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 15882-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15882-1-AP (PPP2R1A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 15882-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15882-1-AP (PPP2R1A antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenlebergewebe, A431-Zellen, HepG2-Zellen, L02-Zellen, Mauslebergewebe, Mausnierengewebe, SMMC-7721-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• CoIP: See 2 publications below

Produktinformation

15882-1-AP bindet in WB, IP, IHC, CoIP, ELISA PPP2R1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP2R1A fusion protein Ag8661
• Vollständiger Name: protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform
• Berechnetes Molekulargewicht: 589 aa, 65 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC001537
• Gene symbol: PPP2R1A
• Gene ID (NCBI): 5518
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPP2R1A belongs to the phosphatase 2A regulatory subunit A family. PPP2R1A encodes the α-isoform of the scaffolding subunit of the serine/threonine protein phosphatase 2A (PP2A) holoenzyme. This tumour suppressor complex is involved in growth and survival pathways and PPP2R1A mutations in endometrial high-grade serous carcinomas are a frequent and potentially targetable feature of this disease(PMID: 21381030). The PPP2R1A gene has 86% identity with the PPP2R1B gene(PMID:7681822).

Publikationen

Species	Application	Title
mouse	WB	Science; Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase.; Authors - Hai Zheng
human	WB	Mol Cell; SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape.; Authors - Shibin Hu
human	WB	Front Cell Dev Biol; BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells.; Authors - Baoyu Chen
human,pig	WB,CoIP	Microbiol Spectr; TurboID Screening of the OmpP2 Protein Reveals Host Proteins Involved in Recognition and Phagocytosis of Glaesserella parasuis by iPAM Cells; Authors - Changsheng Jiang ; KD Validated
human	CoIP	Dev Cell; Fam72a functions as a cell-cycle-controlled gene during proliferation and antagonizes apoptosis through reprogramming PP2A substrates; Authors - Yuan Fu
human,mouse	WB	Cell Death Discov; Venlafaxine antagonizes the noradrenaline-promoted colon cancer progression by inhibiting the norepinephrine transporter; Authors - Huahua Zhang ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Zee (Verified Customer) (01-28-2020)

It worked very well for western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue