PPP2R3A Polyklonaler Antikörper

PPP2R3A Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 14720-1-AP

Synonyme

PPP2R3, PPP2R3A, PR130, PR72

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Galerie der Validierungsdaten

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  WB analysis of PC-3 using 14720-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14720-1-AP (PPP2R3A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14720-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14720-1-AP (PPP2R3A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautgewebe, humanes Eierstockgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

14720-1-AP bindet in WB, IHC, IF, ELISA PPP2R3A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP2R3A fusion protein Ag6450
• Vollständiger Name: protein phosphatase 2 (formerly 2A), regulatory subunit B'', alpha
• Berechnetes Molekulargewicht: 130 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC065531
• Gene symbol: PPP2R3A
• Gene ID (NCBI): 5523
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPP2R3A(Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha), also named as PPP2R3, has 2 isoforms produced by alternative splicing named as PR130 and PR72. The protein contains stretches of basic residues that may be putative nuclear localization signals, and a cluster of proline residues in an N-terminal region common to both isoforms that the isoform PR130 but not PR72 is variably expressed in a number of tumor cell lines. The PR72 and PR130 cDNAs encode 72 and 130 kDa proteins when expressed in rabbit reticulocyte lysates.(PMID:8392071).

Publikationen

Species	Application	Title
human	WB,IF	Cell Biosci; GREM1/PPP2R3A expression in heterogeneous fibroblasts initiates pulmonary fibrosis.; Authors - Xiaoni Shi
human	WB	Cell Signal; PP2A-AMPKα-HSF1 axis regulates metal-induced HSP expression and ROS clearance.; Authors - Xiao-Nian Zhu