PPP2R5A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PPP2R5A Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12675-2-AP

Synonyme

B56A, PP2A B subunit isoform B alpha, PPP2R5A, PR61A, PR61alpha

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Galerie der Validierungsdaten

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  WB analysis of human ileum using 12675-2-AP

  human ileum tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12675-2-AP

  WB result of PPP2R5A antibody (12675-2-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPP2R5A transfected HeLa cells.

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  WB analysis using 12675-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12675-2-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12675-2-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12675-2-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12675-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12675-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12675-2-AP

  IP Result of anti-PPP2R5A (IP:12675-2-AP, 4ug; Detection:12675-2-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human pancreas cancer using 12675-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12675-2-AP (PPP2R5A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12675-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12675-2-AP (PPP2R5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12675-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12675-2-AP (PPP2R5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12675-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12675-2-AP (PPP2R5A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Ileumgewebe, HeLa-Zellen, humanes Herzgewebe, humanes Hirngewebe, humanes Lungengewebe, humanes Nierengewebe, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

12675-2-AP bindet in WB, IP, IHC, IF, ELISA PPP2R5A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP2R5A fusion protein Ag3360
• Vollständiger Name: protein phosphatase 2, regulatory subunit B', alpha isoform
• Berechnetes Molekulargewicht: 486 aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC022474
• Gene symbol: PPP2R5A
• Gene ID (NCBI): 5525
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPP2R5A(protein phosphatase 2, regulatory subunit B (B56), alpha isoform) is also named as PR61alpha and belongs to the phosphatase 2A regulatory subunit B56 family. B56 alpha subunit gene encodes a cytoplasmic phosphoprotein(PMID:7592815). The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 54 kDa and 50 kDa.

Publikationen

Species	Application	Title
human	WB,IHC	Oncol Rep; PMID: 28849187; Authors - Zehang Zhuang ; KD Validated
human	WB	Oncol Lett; PP2A promotes apoptosis and facilitates docetaxel sensitivity via the PP2A/p-eIF4B/XIAP signaling pathway in prostate cancer.; Authors - Siwen Yin
human	WB	Cell Death Dis; Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration; Authors - Xiaoman Zhu