PPP3CB-specific Polyklonaler Antikörper

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• KD/KO Validated

PPP3CB-specific Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 55148-1-AP

Synonyme

CALNA2, CALNB, CAM PRP catalytic subunit, CNA2, PPP3CB, PPP3CB-specific

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Galerie der Validierungsdaten

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  WB analysis using 55148-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55148-1-AP (PPP3CB-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 55148-1-AP

  WB result of PPP3CB-specific antibody (55148-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPP3CB-specific transfected HEK-293 cells.

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  WB analysis using 55148-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55148-1-AP (PPP3CB-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55148-1-AP

  IP Result of anti-PPP3CB-specific (IP:55148-1-AP, 4ug; Detection:55148-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human heart using 55148-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55148-1-AP (PPP3CB-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 55148-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55148-1-AP (PPP3CB-specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 55148-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55148-1-AP (PPP3CB-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 55148-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55148-1-AP (PPP3CB-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 55148-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 55148-1-AP (PPP3CB-specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, fetales humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

55148-1-AP bindet in WB, IP, IHC, IF, ELISA PPP3CB-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: NM_021132
• Gene symbol: PPP3CB
• Gene ID (NCBI): 5532
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPP3CB is one of the three isoforms of the A subunit of calcineurin (CnA). Calcineurin is a calcium-dependent serine/threonine phosphatase. It consists of two subunits: subunit A (CnA) and subunit B (CnB). For CnA, three isoforms PPP3CA (CnAα), PPP3CB (CnAβ) and PPP3CC (CnAγ) have been identified. PPP3CB is a calcium-dependent, calmodulin-stimulated protein phosphatase. It may have a role in the calmodulin activation of calcineurin. This antibody is specific to PPP3CB.

Publikationen

Species	Application	Title
rat, mouse, human	WB,IHC,IF	J Clin Invest; MicroRNA-30 family members regulate calcium/calcineurin signaling in podocytes.; Authors - Junnan Wu
mouse	WB	Mol Psychiatry; A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.; Authors - Yue Qin
mouse	WB,IHC	J Mol Med (Berl); Angiotensin II induces calcium/calcineurin signaling and podocyte injury by downregulating microRNA-30 family members.; Authors - Yue Zhao
human	WB	Cancer Commun (Lond); HMMR alleviates endoplasmic reticulum stress by promoting autophagolysosomal activity during endoplasmic reticulum stress-driven hepatocellular carcinoma progression; Authors - Lin He