PPP6C Polyklonaler Antikörper

PPP6C Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 15852-1-AP

Synonyme

PP6C, PPP6, PPP6C

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Galerie der Validierungsdaten

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  WB analysis using 15852-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15852-1-AP (PPP6C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15852-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15852-1-AP (PPP6C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15852-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15852-1-AP (PPP6C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 15852-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15852-1-AP (PPP6C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 15852-1-AP

  IP Result of anti-PPP6C (IP:15852-1-AP, 3ug; Detection:15852-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of mouse testis using 15852-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15852-1-AP (PPP6C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15852-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15852-1-AP (PPP6C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, HEK-293-Zellen, humanes Hodengewebe, NIH/3T3-Zellen, Rattenhodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Produktinformation

15852-1-AP bindet in WB, IP, IHC, CoIP, ELISA PPP6C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP6C fusion protein Ag8670
• Vollständiger Name: protein phosphatase 6, catalytic subunit
• Berechnetes Molekulargewicht: 305 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC006990
• Gene symbol: PPP6C
• Gene ID (NCBI): 5537
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,CoIP	Protein Cell; Dephosphorylation of cGAS by PPP6C impairs its substrate binding activity and innate antiviral response.; Authors - Mi Li
		J Biol Chem; Leucine Carboxyl Methyltransferase 1 (LCMT-1) Methylates Protein Phosphatase 4 (PP4) and Protein Phosphatase 6 (PP6) and Differentially Regulates the Stable Formation of Different PP4 Holoenzymes.; Authors - Juyeon Hwang
human	WB,IP	Cell Death Dis; PP6 negatively modulates LUBAC-mediated M1-ubiquitination of RIPK1 and c-FLIPL to promote TNFα-mediated cell death; Authors - Guowei Wu ; KO Validated
mouse,human	WB,IP,CoIP	Mol Cell; Energy sensor AMPK gamma regulates translation via phosphatase PPP6C independent of AMPK alpha; Authors - Qi Zhou
canine	WB	J Vet Med Sci; Protein phosphatase 6 regulates trametinib sensitivity, a mitogen-activated protein kinase kinase (MEK) inhibitor, by regulating MEK1/2-ERK1/2 signaling in canine melanoma cells; Authors - Miu Yamamoto ; KD Validated