PPT1 Monoklonaler Antikörper

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• KD/KO Validated

PPT1 Monoklonal Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Kaninchen

Anwendung

WB, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A1A3

Kat-Nr. : 67699-1-Ig

Synonyme

Palmitoyl-protein thioesterase 1, Palmitoyl-protein hydrolase 1, INCL, EC:3.1.2.22, CLN1

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  WB analysis using 67699-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67699-1-Ig (PPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67699-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67699-1-Ig (PPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 67699-1-Ig

  WB result of PPT1 antibody (67699-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPT1 transfected A549 cells.

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  WB analysis of pig heart using 67699-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67699-1-Ig (PPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse testis using 67699-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using PPT1 antibody (67699-1-Ig, Clone: 1A1A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, Hausschwein-Hirngewebe, Hausschwein-Cerebellum-Gewebe, Hausschwein-Herzgewebe, Kaninchenhirngewebe, Kaninchen-Cerebellum-Gewebe
• Erfolgreiche Detektion in IF-P: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67699-1-Ig bindet in WB, IF-P, ELISA PPT1 und zeigt Reaktivität mit Hausschwein, human, Kaninchen

• Getestete Reaktivität: Hausschwein, human, Kaninchen
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PPT1 fusion protein Ag30550
• Vollständiger Name: palmitoyl-protein thioesterase 1
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 32-34 kda
• GenBank-Zugangsnummer: BC008426
• Gene symbol: PPT1
• Gene ID (NCBI): 5538
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The defective gene behind the INCL(infantile neuronal ceroid lipofuscinoses) disease, CLN1, encodes for palmitoyl protein thioesterase 1 (PPT1). It consists of 306 amino acids, including a signal sequence of 26 amino acids and three N-linked glycosylation sites. This protein exists with a molecular mass of 32 kDa, 34 kDa,36 kDa, and 38 kDa. The enzyme is transported into lysosomes of non-neuronal cells by the mannose 6-phosphate receptor (M6PR) mediated pathway and a significant amount of native PPT1 resides in a complex rather than in a monomeric form(PMID:17565660).