PRAP1 Polyklonaler Antikörper

PRAP1 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 11932-1-AP

Synonyme

PRAP1, PRO1195, proline rich acidic protein 1, RP11 122K13.6, UPA

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Galerie der Validierungsdaten

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  WB analysis of L02 using 11932-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11932-1-AP (PRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 11932-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11932-1-AP (PRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 11932-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11932-1-AP (PRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 11932-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11932-1-AP (PRAP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 11932-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11932-1-AP (PRAP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 11932-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11932-1-AP (PRAP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse small intestine using 11932-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 11932-1-AP (PRAP1 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of mouse small intestine using 11932-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 11932-1-AP (PRAP1 Antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Nierengewebe, Maus-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

11932-1-AP bindet in WB, IHC, ELISA PRAP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRAP1 fusion protein Ag2526
• Vollständiger Name: proline-rich acidic protein 1
• Berechnetes Molekulargewicht: 151 aa, 17 kDa
• Beobachtetes Molekulargewicht: 16-20 kDa
• GenBank-Zugangsnummer: BC029447
• Gene symbol: PRAP1
• Gene ID (NCBI): 118471
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Proline rich acidic protein 1 (PRAP1) is also named as PRO1195 or UPA. It may play an important role in maintaining normal growth homeostasis in epithelial cells. Mitotic arrest deficient 1 (MAD1) is a key component in mitotic checkpoint signalling. Recent study identified PRAP1 as a novel MAD1 binding partner using yeast-two hybrid screening (PMID: 24374861). PRAP1 has four isoforms with MW 16-20 kDa and 10 kDa.

Publikationen

Species	Application	Title
human	IHC,WB	Adv Sci (Weinh); Adipocyte-Derived Exosomal MTTP Suppresses Ferroptosis and Promotes Chemoresistance in Colorectal Cancer; Authors - Qiumo Zhang ; KD Validated