PREB Monoklonaler Antikörper

PREB Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2F6A1

Kat-Nr. : 66981-1-Ig

Synonyme

2F6A1, Guanine nucleotide-exchange factor SEC12, Prolactin regulatory element-binding protein, SEC12

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  WB analysis of Daudi using 66981-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66981-1-Ig (PREB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Ramos using 66981-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66981-1-Ig (PREB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 66981-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66981-1-Ig (PREB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 66981-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66981-1-Ig (PREB antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66981-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66981-1-Ig (PREB antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, Raji-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66981-1-Ig bindet in WB, IHC, ELISA PREB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PREB fusion protein Ag28549
• Vollständiger Name: prolactin regulatory element binding
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC002765
• Gene symbol: PREB
• Gene ID (NCBI): 10113
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PREB contains two PQ-rich potential transactivation domains, but no apparent DNA-binding motif, and exhibits sequence-specific binding to Pit1-binding element of the prolactin (PRL) promoter. It may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p.