PRKAR2A Monoklonaler Antikörper

PRKAR2A Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B2B3

Kat-Nr. : CL488-67751

Synonyme

PRKAR2, PKR2, cAMP-dependent protein kinase type II-alpha regulatory subunit, 1B2B3

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  IF Staining of HeLa using CL488-67751

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 PRKAR2A antibody (CL488-67751, Clone: 1B2B3 ) at dilution of 1:200.

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  FC experiment of MCF-7 using CL488-67751

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PRKAR2A (CL488-67751, Clone:1B2B3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67751 bindet in IF/ICC, FC (Intra) PRKAR2A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PRKAR2A fusion protein Ag29893
• Vollständiger Name: protein kinase, cAMP-dependent, regulatory, type II, alpha
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC002763
• Gene symbol: PRKAR2A
• Gene ID (NCBI): 5576
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory and two catalytic subunits. The regulatory subunit of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2. The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy.