PKC Epsilon Polyklonaler Antikörper

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• KD/KO Validated

PKC Epsilon Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 20877-1-AP

Synonyme

PKC epsilon, PKCE, PRKCE, Protein kinase C epsilon type, protein kinase C, epsilon

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Galerie der Validierungsdaten

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  WB analysis using 20877-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20877-1-AP (PKC Epsilon antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 20877-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20877-1-AP (PKC epsilon antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 20877-1-AP

  IP result of anti-PKC Epsilon(IP:20877-1-AP, 4ug; Detection:20877-1-AP 1:800) with SH-SY5Y cells lysate 1240 ug.

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  IP experiment of SH-SY5Y using 20877-1-AP

  IP result of anti-PKC Epsilon(IP:20877-1-AP, 4ug; Detection:20877-1-AP 1:20000) with SH-SY5Y cells lysate 1240 ug.

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  IHC staining of mouse brain using 20877-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20877-1-AP (PKC epsilon antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of mouse brain using 20877-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20877-1-AP (PKC epsilon antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human thyroid cancer using 20877-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 20877-1-AP (PKC epsilon antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human thyroid cancer using 20877-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 20877-1-AP (PKC epsilon antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 20877-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKC Epsilon antibody (20877-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of U2OS using 20877-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using PKC Epsilon antibody (20877-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of mouse brain using 20877-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PKC Epsilon antibody (20877-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 20877-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PKC Epsilon antibody (20877-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Jurkat using 20877-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human PKC Epsilon (20877-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe, HeLa-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

20877-1-AP bindet in WB, IP, IHC, IF, FC, ELISA PKC Epsilon und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PKC Epsilon fusion protein Ag14976
• Vollständiger Name: protein kinase C, epsilon
• Berechnetes Molekulargewicht: 737 aa, 84 kDa
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC109033
• Gene symbol: PRKCE
• Gene ID (NCBI): 5581
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF	Elife; cAMP-EPAC-PKCε-RIM1α signaling regulates presynaptic long-term potentiation and motor learning; Authors - Xin-Tai Wang
mouse	WB	Biochem Pharmacol; Thromboxane A2-TP axis promotes adipose tissue macrophages M1 polarization leading to insulin resistance in obesity; Authors - Ruijie Xu
mouse	WB	J Cell Mol Med; Protein kinase C is involved in the neuroprotective effect of berberine against intrastriatal injection of quinolinic acid-induced biochemical alteration in mice.; Authors - Peng Liu
	WB	Fish Shellfish Immunol; In vivo study of a novel protein kinase C that mediates immunocompetence and catecholamine biosynthesis in hemocytes of Litopenaeus vannamei by using its potential competitive inhibitor, bisindolylmaleimide I.; Authors - Zhong-Wen Chang
human	WB	Mol Biol Cell; MiR-34a regulates blood-tumor barrier function by targeting protein kinase Cɛ.; Authors - Wei Zhao
human	WB	Oxid Med Cell Longev; p66Shc Mediates Mitochondrial Dysfunction Dependent on PKC Activation in Airway Epithelial Cells Induced by Cigarette Smoke.; Authors - Ming Zhang