PKC Epsilon Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PKC Epsilon Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(22)

Kat-Nr. : 20877-1-AP

Synonyme

PRKCE, Protein kinase C epsilon type, PKCE, nPKC-epsilon, EC:2.7.11.13

---

• 

  WB analysis using 20877-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20877-1-AP (PKC Epsilon antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 20877-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20877-1-AP (PKC epsilon antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 20877-1-AP

  IP result of anti-PKC Epsilon (IP:20877-1-AP, 4ug; Detection:20877-1-AP 1:800) with SH-SY5Y cells lysate 1240 ug.

• 

  IP experiment of SH-SY5Y using 20877-1-AP

  IP result of anti-PKC Epsilon (IP:20877-1-AP, 4ug; Detection:20877-1-AP 1:20000) with SH-SY5Y cells lysate 1240 ug.

• 

  IHC staining of mouse brain using 20877-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20877-1-AP (PKC epsilon antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of mouse brain using 20877-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20877-1-AP (PKC epsilon antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human thyroid cancer using 20877-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 20877-1-AP (PKC epsilon antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human thyroid cancer using 20877-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 20877-1-AP (PKC epsilon antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of mouse brain using 20877-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PKC Epsilon antibody (20877-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 20877-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PKC Epsilon antibody (20877-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 20877-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKC Epsilon antibody (20877-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of U2OS using 20877-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using PKC Epsilon antibody (20877-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of U2OS using 20877-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PKC Epsilon antibody (20877-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 20877-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human PKC Epsilon (20877-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 20 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

20877-1-AP bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA PKC Epsilon und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PKC Epsilon fusion protein Ag14976
• Vollständiger Name: protein kinase C, epsilon
• Berechnetes Molekulargewicht: 737 aa, 84 kDa
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC109033
• Gene symbol: PKC Epsilon
• Gene ID (NCBI): 5581
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PKC Epsilon (Protein kinase C epsilon type) is also named as PKC-ε, PRKCE and PKCE. It belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and PKC subfamily. PKC-ε, one of the novel isoforms, as a critical player in membrane mobilization during phagocytosis (PMID: 9069266). KC-ε is tethered to the Golgi through binding of its pseudosubstrate domain to phosphatidylinositol-4-phosphate (PI4P) (PMID: 28539432). PKC-ε traffics on microtubule-associated vesicles from the Golgi to the forming phagosome. As the majority of macrophage PKC-ε is cytosolic, and PKCs translocate from the cytosol to their sites of activity, it predicted that cytosolic PKC-ε concentrated at phagosomes to facilitate membrane addition (PMID: 34622926). Two PKC isozymes of the novel group, PKCε and PKCδ, have different and sometimes opposite effects. PKCε stimulates cell growth and differentiation while PKCδ is apoptotic (PMID: 21810427).

Publikationen

Species	Application	Title
mouse	IF	Elife; cAMP-EPAC-PKCε-RIM1α signaling regulates presynaptic long-term potentiation and motor learning; Authors - Xin-Tai Wang
mouse	WB	Biochem Pharmacol; Thromboxane A2-TP axis promotes adipose tissue macrophages M1 polarization leading to insulin resistance in obesity; Authors - Ruijie Xu
mouse	WB	J Cell Mol Med; Protein kinase C is involved in the neuroprotective effect of berberine against intrastriatal injection of quinolinic acid-induced biochemical alteration in mice.; Authors - Peng Liu
	WB	Fish Shellfish Immunol; In vivo study of a novel protein kinase C that mediates immunocompetence and catecholamine biosynthesis in hemocytes of Litopenaeus vannamei by using its potential competitive inhibitor, bisindolylmaleimide I.; Authors - Zhong-Wen Chang
human	WB	Mol Biol Cell; MiR-34a regulates blood-tumor barrier function by targeting protein kinase Cɛ.; Authors - Wei Zhao
human	WB	Oxid Med Cell Longev; p66Shc Mediates Mitochondrial Dysfunction Dependent on PKC Activation in Airway Epithelial Cells Induced by Cigarette Smoke.; Authors - Ming Zhang