PRKCSH Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PRKCSH Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 12148-1-AP

Synonyme

PCLD, Glucosidase II subunit beta, Glucosidase 2 subunit beta, G19P1, 80K-H protein

---

• 

  WB analysis using 12148-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12148-1-AP (PRKCSH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12148-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12148-1-AP (PRKCSH antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12148-1-AP

  IP result of anti-PRKCSH (IP:12148-1-AP, 4ug; Detection:12148-1-AP 1:1000) with HeLa cells lysate 2000ug.

• 

  IHC staining of human kidney using 12148-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12148-1-AP (PRKCSH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 12148-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12148-1-AP (PRKCSH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 12148-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12148-1-AP (PRKCSH antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12148-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12148-1-AP (PRKCSH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, Jurkat-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, human normal colon; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:1600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

12148-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PRKCSH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRKCSH fusion protein Ag2796
• Vollständiger Name: protein kinase C substrate 80K-H
• Berechnetes Molekulargewicht: 80 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC013586
• Gene symbol: PRKCSH
• Gene ID (NCBI): 5589
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRKCSH encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Defects in PRKCSH are a cause of an autosomal dominant polycystic liver disease (PCLD). Glucosidase II is an ER-localized enzyme that contains α and β subunits (Glucosidase IIα and Glucosidase IIβ) which form a defined heterodimeric complex.

Publikationen

Species	Application	Title
human	WB	Exp Mol Med; PRKCSH contributes to TNFSF resistance by extending IGF1R half-life and activation in lung cancer; Authors - Gu-Choul Shin
human	WB	Nat Commun; PRKCSH contributes to tumorigenesis by selective boosting of IRE1 signaling pathway.; Authors - Gu-Choul Shin ; KD Validated
rat	WB	J Cell Biol; Competition for calnexin binding regulates secretion and turnover of misfolded GPI-anchored proteins; Authors - Amber M Cheatham
mouse	IHC	Exp Physiol; AGEs-RAGE axis mediates myocardial fibrosis via activation of cardiac fibroblasts induced by autophagy in heart failure.; Authors - Birong Liang
human	IHC	Cell Rep; Spatial region-resolved proteome map reveals mechanism of COVID-19-associated heart injury.; Authors - Ling Leng
human	IF	Biochim Biophys Acta; Hepatocystin/80K-H inhibits replication of hepatitis B virus through interaction with HBx protein in hepatoma cell.; Authors - Shin Gu-Choul GC