PROS1 Polyklonaler Antikörper

PROS1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Rezensionen (1)

Kat-Nr. : 16910-1-AP

Synonyme

PROS, Vitamin K-dependent protein S

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• 

  WB analysis of human placenta using 16910-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16910-1-AP (PROS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16910-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16910-1-AP (PROS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 16910-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 16910-1-AP (PROS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 16910-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16910-1-AP (PROS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 16910-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16910-1-AP (PROS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16910-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16910-1-AP (PROS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16910-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PROS1 antibody (16910-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

• 

  FC experiment of HepG2 using 16910-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PROS1 (16910-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, DU 145-Zellen, HepG2-Zellen, HuH-7-Zellen, Maus-Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• ELISA: See 1 publications below

Produktinformation

16910-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA PROS1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Plasmodium falciparum
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PROS1 fusion protein Ag10539
• Vollständiger Name: protein S (alpha)
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC015801
• Gene symbol: PROS1
• Gene ID (NCBI): 5627
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PROS1, also named as Vitamin K-dependent protein S, is a 676 amino acid protein, which contains 4 EGF-like domains, contains one Gla (gamma-carboxy-glutamate) domain and contains 2 laminin G-like domains. PROS1 as a secreted protein platelets alpha granules is expressed in plasma. PROS1 is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa and helps to prevent coagulation and stimulating fibrinolysis.

Publikationen

Species	Application	Title
mouse		Science; The TAM family receptor tyrosine kinase TYRO3 is a negative regulator of type 2 immunity.; Authors - Pamela Y Chan
		Brain; Extracellular vesicle biomarkers for complement dysfunction in schizophrenia; Authors - Ting Xue
human	WB,IHC	Aging (Albany NY); CeRNA regulatory network-based analysis to study the roles of noncoding RNAs in the pathogenesis of intrahepatic cholangiocellular carcinoma.; Authors - Weiyu Xu
human	WB	Mol Cell Proteomics; Multisample Mass Spectrometry-Based Approach for Discovering Injury Markers in Chronic Kidney Disease.; Authors - Ji Eun Kim
mouse	WB	Front Cell Neurosci; Proteomic and Metabolomic Analyses of Vanishing White Matter Mouse Astrocytes Reveal Deregulation of ER Functions.; Authors - Lisanne E Wisse
mouse	IF	Am J Physiol Renal Physiol; Identification of natriuretic peptide receptor A-related gene expression signatures in podocytes in vivo reveals baseline control of protective pathways; Authors - Mia Jensen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hongxue (Verified Customer) (01-25-2021)

Nice band for WB

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Liver

PROS1 Antibody Western Blot validation (1:1000 dilution) in Liver (Cat no:16910-1-AP)