PRPH2 Polyklonaler Antikörper

PRPH2 Polyklonal Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(20)

Rezensionen (1)

Kat-Nr. : 18109-1-AP

Synonyme

PRPH, Peripherin-2, Peripherin2, Peripherin 2, AVMD

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  WB analysis of mouse eye using 18109-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 18109-1-AP (PRPH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse eye using 18109-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRPH2 antibody (18109-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Augengewebe
• Erfolgreiche Detektion in IF-P: Maus-Augengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 5 publications below
• IF: See 11 publications below
• IP: See 1 publications below

Produktinformation

18109-1-AP bindet in WB, IHC, IF-P, IP, ELISA PRPH2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRPH2 fusion protein Ag12555
• Vollständiger Name: peripherin 2 (retinal degeneration, slow)
• Berechnetes Molekulargewicht: 346 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC074720
• Gene symbol: PRPH2
• Gene ID (NCBI): 5961
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Peripherin-2 (PRPH2), also known as retinal degeneration slow protein (RDS), is a photoreceptor-specific tetraspanin protein implicated in outer segment disk morphogenesis. It may function as an adhesion molecule involved in stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. Mutations in peripherin-2 are responsible for various retinal degenerative diseases including autosomal dominant retinitis pigmentosa (ADRP).

Publikationen

Species	Application	Title
		Biomaterials; Hydrogel-mediated co-transplantation of retinal pigmented epithelium and photoreceptors restores vision in an animal model of advanced retinal degeneration.; Authors - Nikolaos Mitrousis
mouse	WB, IF	Proc Natl Acad Sci U S A; Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.; Authors - Poppy Datta
mouse	IHC	Proc Natl Acad Sci U S A; Reversal of end-stage retinal degeneration and restoration of visual function by photoreceptor transplantation.; Authors - Singh Mandeep S MS
human	IHC	Proc Natl Acad Sci U S A; Transplantation of human embryonic stem cell-derived retinal tissue in two primate models of retinal degeneration.; Authors - Hiroshi Shirai
mouse	IF	Cell Death Dis; Prph2 knock-in mice recapitulate human central areolar choroidal dystrophy retinal degeneration and exhibit aberrant synaptic remodeling and microglial activation; Authors - María José Ruiz-Pastor
mouse	IHC	Hum Mol Genet; BBS mutations modify phenotypic expression of CEP290-related ciliopathies.; Authors - Zhang Yan Y

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Melina (Verified Customer) (05-13-2024)

Worked very well in western blots of whole retina lysate

• Applications: Western Blot
• Primary Antibody Dilution: 1 ug/ml
• Cell Tissue Type: retina

PRPH2 Antibody Western Blot validation (1 ug/ml dilution) in retina (Cat no:18109-1-AP)