PSCA Polyklonaler Antikörper

PSCA Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 17171-1-AP

Synonyme

PRO232, prostate stem cell antigen, PSCA

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  WB analysis of PC-3 using 17171-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 17171-1-AP (PSCA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 17171-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17171-1-AP (PSCA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Maus-Skelettmuskelgewebe, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

17171-1-AP bindet in WB, ELISA PSCA und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSCA fusion protein Ag10710
• Vollständiger Name: prostate stem cell antigen
• Berechnetes Molekulargewicht: 123 aa, 13 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC023582
• Gene symbol: PSCA
• Gene ID (NCBI): 8000
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Prostate stem cell antigen (PSCA) is a protein composed of 123 amino acid residues. PSCA belongs to the LY-6/Thy-1 family of cell surface antigens. It is highly expressed in normal prostate and further up-regulated in prostate cancer, as well as non-prostatic malignancies including gastric cancer. PSCA plays a critical role in cell adhesion, proliferation, and survival. The obasrved MW of PSCA is between 36 to 40 kDa in paper (PMID: 11309275).

Publikationen

Species	Application	Title
mouse	WB	Endocrinology; mTOR regulates mineralocorticoid receptor transcriptional activity by ULK1- dependent and independent mechanisms; Authors - Yusuf Ali
human	WB	PLoS One; Pan-cancer analysis of PSCA that is associated with immune infiltration and affects patient prognosis; Authors - Chenxing Wang ; KD Validated
mouse	WB	J Immunother Cancer; Impact of tumor localization on antitumor immunity with conditionally activated CTLA-4 blockade; Authors - Marcel Arias-Badia