Presenilin-1-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Presenilin-1-Specific Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Rezensionen (1)

Kat-Nr. : 16163-1-AP

Synonyme

AD3, FAD, Presenilin-1, Presenilin-1-Specific, Protein S182, PS 1, PS1, PS1 CTF12, PSEN1, PSNL1, S182

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Galerie der Validierungsdaten

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  WB analysis using 16163-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16163-1-AP (Presenilin-1-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16163-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16163-1-AP (Presenilin-1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 16163-1-AP

  IP Result of anti-Presenilin-1-Specific (IP:16163-1-AP, 4ug; Detection:16163-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 16163-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16163-1-AP (Presenilin-1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16163-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16163-1-AP (Presenilin-1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 16163-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16163-1-AP (Presenilin-1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COS-7-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 1 publications below

Produktinformation

16163-1-AP bindet in WB, IP, IHC, ELISA Presenilin-1-Specific und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: presenilin 1
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 21kDa
• GenBank-Zugangsnummer: NM_000021
• Gene symbol: PSEN1
• Gene ID (NCBI): 5663
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSEN1(presenilin-1) is also named as AD3, PS1, PSNL1 and belongs to the peptidase A22A family. It directly influences Netrin/DCC signaling with cell type precision and spatiotemporal specificity, and is a key neural circuit builder that gates the spatiotemporal pattern of guidance signaling, thereby ensuring neural projections occur with high fidelity(PMID:21215373). The 46-kDa PSEN1 holoprotein is cleaved into a D30-kDa N-terminal fragment (NTF) and a D20-kDa C-terminal fragment (CTF) by the 26S proteasome(PMID:10527805). It has 7 isoforms produced by alternative splicing. This antibody is specific to isoform1(53 kDa), isoform2(52 kDa), isoform4(21 kDa), isoform7(49 kDa) of this protein.

Publikationen

Species	Application	Title
mouse	WB	Adv Sci (Weinh); Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression; Authors - Rui Zhou
human	WB	Brain; MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.; Authors - Bing-Lin Zhu
human,mouse	WB	Cell Rep; CIP2A Causes Tau/APP Phosphorylation, Synaptopathy, and Memory Deficits in Alzheimer's Disease.; Authors - Yang-Ping Shentu
mouse	WB	J Neuroinflammation; Far infrared light irradiation enhances Aβ clearance via increased exocytotic microglial ATP and ameliorates cognitive deficit in Alzheimer's disease-like mice.; Authors - Qingyong Li
human	WB	Cell Commun Signal; Presenilin1 exerts antiproliferative effects by repressing the Wnt/β-catenin pathway in glioblastoma.; Authors - Wei Yang ; KD Validated
mouse	WB	Mol Neurobiol; Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression.; Authors - Yasuteru Inoue

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xiaoyu (Verified Customer) (02-07-2024)

Good signal

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: brain

Presenilin-1-Specific Antibody Western Blot validation (1:1000 dilution) in brain (Cat no:16163-1-AP)