PSG11 Polyklonaler Antikörper

PSG11 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 16352-1-AP

Synonyme

Pregnancy-specific beta-1-glycoprotein 11, Pregnancy-specific beta-1-glycoprotein 13, Pregnancy-specific glycoprotein 11, Pregnancy-specific glycoprotein 13, PS beta G 11

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  WB analysis of mouse uterus using 16352-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 16352-1-AP (PSG11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16352-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16352-1-AP (PSG11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 16352-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSG11 antibody (16352-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Uterusgewebe, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Nierengewebe, humanes Lebergewebe, humanes Lungengewebe, humanes Plazenta-Gewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

16352-1-AP bindet in WB, IHC, IF/ICC, ELISA PSG11 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSG11 fusion protein Ag9562
• Vollständiger Name: pregnancy specific beta-1-glycoprotein 11
• Berechnetes Molekulargewicht: 219 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC020711
• Gene symbol: PSG11
• Gene ID (NCBI): 5680
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSG11, also named as PSG13, PSG14, PSBG-11 and PSBG-13, belongs to the immunoglobulin superfamily and CEA family. PSBG are produced in high quantity during pregnancy. PSG11 and other PSGs induce dose-dependent secretion of anti-inflammatory cytokines by human monocytes. (PMID:11327547)

Publikationen

Species	Application	Title
	WB	Front Genet; Telomeric Double Strand Breaks in G1 Human Cells Facilitate Formation of 5' C-Rich Overhangs and Recruitment of TERRA.; Authors - Christopher B Nelson