PSMA4 Polyklonaler Antikörper

PSMA4 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11943-2-AP

Synonyme

20S proteasome, HC9, HsT17706, Macropain subunit C9, Proteasome component C9, Proteasome subunit L, PSC9, PSMA4

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Galerie der Validierungsdaten

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  WB analysis using 11943-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11943-2-AP (PSMA4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 11943-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11943-2-AP (PSMA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 11943-2-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11943-2-AP (PSMA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of BxPC-3 using 11943-2-AP

  IP Result of anti-PSMA4 (IP:11943-2-AP, 4ug; Detection:11943-2-AP 1:500) with BxPC-3 cells lysate 2800ug.

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  IHC staining of mouse liver using 11943-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11943-2-AP (PSMA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 11943-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11943-2-AP (PSMA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11943-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11943-2-AP (PSMA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11943-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11943-2-AP (PSMA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11943-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11943-2-AP (PSMA4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, K-562-Zellen, Mausmilzgewebe, Mausnierengewebe, U-937-Zellen
• Erfolgreiche IP: BxPC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

11943-2-AP bindet in WB, IP, IHC, IF, ELISA PSMA4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMA4 fusion protein Ag2542
• Vollständiger Name: proteasome (prosome, macropain) subunit, alpha type, 4
• Berechnetes Molekulargewicht: 237 aa, 27 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC056249
• Gene symbol: PSMA4
• Gene ID (NCBI): 5685
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IHC	J Biol Chem; PITHD1 is a proteasome-interacting protein essential for male fertilization.; Authors - Hiroyuki Kondo
mouse	IHC	Sci Rep; Proteasome inhibition by bortezomib parallels a reduction in head and neck cancer cells growth, and an increase in tumor-infiltrating immune cells.; Authors - Monica Benvenuto
human	WB	PLoS One; Inactive USP14 and inactive UCHL5 cause accumulation of distinct ubiquitinated proteins in mammalian cells.; Authors - Jayashree Chadchankar
human	WB	Cell Death Differ; Quantitative reactive cysteinome profiling reveals a functional link between ferroptosis and proteasome-mediated degradation; Authors - Yankun Wang