PSMA6 Polyklonaler Antikörper

PSMA6 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11573-1-AP

Synonyme

20S proteasome, 27 kDa prosomal protein, IOTA, Macropain iota chain, p27K, PROS 27, PROS27, Proteasome iota chain, PSMA6

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Galerie der Validierungsdaten

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  WB analysis using 11573-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11573-1-AP (PSMA6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11573-1-AP

  IP Result of anti-PSMA6 (IP:11573-1-AP, 4ug; Detection:11573-1-AP 1:1000) with HEK-293 cells lysate 3200ug.

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  IHC staining of human lung cancer using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11573-1-AP (PSMA6 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11573-1-AP (PSMA6 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of rat small intestine using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 11573-1-AP (PSMA6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11573-1-AP (PSMA6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11573-1-AP (PSMA6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11573-1-AP (PSMA6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11573-1-AP (PSMA6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11573-1-AP (PSMA6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11573-1-AP (PSMA6 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11573-1-AP (PSMA6 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 11573-1-AP

  Immunofluorescent analysis of HeLa cells using 11573-1-AP (PSMA6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 11573-1-AP

  Immunofluorescent analysis of MCF-7 cells using 11573-1-AP (PSMA6 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 11573-1-AP

  Immunofluorescent analysis of HepG2 cells using 11573-1-AP (PSMA6 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 11573-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human PSMA6 (11573-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen, Neuro-2a-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Hodengewebe, humanes Schilddrüsenkarzinomgewebe, Maushodengewebe, Maus-Kolongewebe, Ratten-Dünndarmgewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

11573-1-AP bindet in WB, IP, IHC, IF, FC, ELISA PSMA6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Auster
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMA6 fusion protein Ag2154
• Vollständiger Name: proteasome (prosome, macropain) subunit, alpha type, 6
• Berechnetes Molekulargewicht: 246 aa, 27 kDa
• Beobachtetes Molekulargewicht: 27-29 kDa
• GenBank-Zugangsnummer: BC023659
• Gene symbol: PSMA6
• Gene ID (NCBI): 5687
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMA6(Proteasome subunit alpha type-6) is also named as PROS27 and belongs to the peptidase T1A family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It also has an ATP-dependent proteolytic activity.

Publikationen

Species	Application	Title
human	WB,IF	Small; Inherent Capability of Self-Assembling Nanostructures in Specific Proteasome Activation for Cancer Cell Pyroptosis; Authors - Qian-Wei Luo
oyster	WB,IF	Proteomics; A comparative proteomic analysis reveals important proteins for the fertilization and early embryonic development of the oyster Crassostrea gigas.; Authors - Xiaohong Huang

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C