PSMA7 Polyklonaler Antikörper

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• KD/KO Validated

PSMA7 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Rezensionen (1)

Kat-Nr. : 15219-1-AP

Synonyme

C6, HSPC, Proteasome subunit alpha type-7, Proteasome subunit RC6-1, RC6 1

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  WB analysis of HepG2 using 15219-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15219-1-AP (PSMA7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15219-1-AP

  WB result of PSMA7 antibody (15219-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA7 transfected HeLa cells.

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  IP experiment of mouse brain using 15219-1-AP

  IP result of anti-PSMA7 (IP:15219-1-AP, 4ug; Detection:15219-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human liver cancer using 15219-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15219-1-AP (PSMA7 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15219-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15219-1-AP (PSMA7 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15219-1-AP (PSMA7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15219-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA7 antibody (15219-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 15219-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PSMA7 Polyclonal antibody (15219-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:3000-1:15000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below

Produktinformation

15219-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PSMA7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMA7 fusion protein Ag7389
• Vollständiger Name: proteasome (prosome, macropain) subunit, alpha type, 7
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC004427
• Gene symbol: PSMA7
• Gene ID (NCBI): 5688
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMA7(Proteasome subunit alpha type-7) is also named HSPC and belongs to the peptidase T1A family. It is an α-type subunit of the 20S proteasome core complex and participates in degrading proteins through the ubiquitin-proteasome pathway (UPP) which plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis(PMID: 19442227).

Publikationen

Species	Application	Title
mouse	WB	Brain; Raising cGMP restores proteasome function and myelination in mice with a proteotoxic neuropathy.; Authors - Jordan J S VerPlank
mouse	WB	Glia; Impairment of protein degradation and proteasome function in hereditary neuropathies.; Authors - Jordan J S VerPlank
human	WB	Mol Cell Proteomics; Proteaphagy in Mammalian Cells Can Function Independent of ATG5/ATG7.; Authors - Tatjana Goebel
mouse	WB	Sci Rep; Starvation-induced metabolic rewiring affects mTORC1 composition in vivo; Authors - Kaade Edgar
human	WB	Biochim Biophys Acta Mol Basis Dis; Defective proteasome biogenesis into skin fibroblasts isolated from Rett syndrome subjects with MeCP2 non-sense mutations.; Authors - Diego Sbardella
human	WB	Cell Physiol Biochem; PSMA7 Directly Interacts with NOD1 and Regulates its Function.; Authors - Yang Liuzhong L

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Janelle (Verified Customer) (09-12-2022)

Specific to target

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000