PSMA7 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

PSMA7 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1F2C6

Kat-Nr. : 67817-1-Ig

Synonyme

C6, HSPC, Proteasome subunit alpha type-7, Proteasome subunit RC6-1, RC6 1

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  WB analysis using 67817-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67817-1-Ig (PSMA7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of MCF-7 using 67817-1-Ig

  WB result of PSMA7 antibody (67817-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA7 transfected MCF-7 cells.

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  WB analysis of HeLa using 67817-1-Ig

  WB result of PSMA7 antibody (67817-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA7 transfected HeLa cells.

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  IHC staining of human cervical cancer using 67817-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67817-1-Ig (PSMA7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 67817-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67817-1-Ig (PSMA7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Caco-2 using 67817-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PSMA7 antibody (67817-1-Ig, Clone: 1F2C6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  IF Staining of MCF-7 using 67817-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PSMA7 antibody (67817-1-Ig, Clone: 1F2C6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  FC experiment of HeLa using 67817-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug PSMA7 Monoclonal antibody (67817-1-Ig, Clone:1F2C6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen, Caco-2-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67817-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), ELISA PSMA7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PSMA7 fusion protein Ag7821
• Vollständiger Name: proteasome (prosome, macropain) subunit, alpha type, 7
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC004427
• Gene symbol: PSMA7
• Gene ID (NCBI): 5688
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMA7(Proteasome subunit alpha type-7) is also named HSPC and belongs to the peptidase T1A family. It is an α-type subunit of the 20S proteasome core complex and participates in degrading proteins through the ubiquitin-proteasome pathway (UPP) which plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis(PMID: 19442227).

Publikationen

Species	Application	Title
human	WB	Heliyon; PSMA7 promotes the malignant proliferation of esophageal cancer; Authors - Qing-Hua Jiao