PSMA7 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

PSMA7 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F2C6

Kat-Nr. : 67817-1-Ig

Synonyme

C6, HSPC, Proteasome subunit RC6 1, Proteasome subunit XAPC7, PSMA7, RC6 1, XAPC7

---

Galerie der Validierungsdaten

• 

  WB analysis using 67817-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67817-1-Ig (PSMA7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis of MCF-7 using 67817-1-Ig

  WB result of PSMA7 antibody (67817-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA7 transfected MCF-7 cells.

• 

  WB analysis of HeLa using 67817-1-Ig

  WB result of PSMA7 antibody (67817-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA7 transfected HeLa cells.

• 

  IHC staining of human cervical cancer using 67817-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67817-1-Ig (PSMA7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 67817-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67817-1-Ig (PSMA7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67817-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PSMA7 antibody (67817-1-Ig, Clone: 1F2C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67817-1-Ig bindet in WB, IHC, IF, ELISA PSMA7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PSMA7 fusion protein Ag7821
• Vollständiger Name: proteasome (prosome, macropain) subunit, alpha type, 7
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC004427
• Gene symbol: PSMA7
• Gene ID (NCBI): 5688
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMA7(Proteasome subunit alpha type-7) is also named HSPC and belongs to the peptidase T1A family. It is an α-type subunit of the 20S proteasome core complex and participates in degrading proteins through the ubiquitin-proteasome pathway (UPP) which plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis(PMID: 19442227).