PSMA8 Monoklonaler Antikörper

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• KD/KO Validated

PSMA8 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

2G2G3

Kat-Nr. : 68123-1-Ig

Synonyme

PSMA7L, PSMA8

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  WB analysis using 68123-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68123-1-Ig (PSMA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lung using 68123-1-Ig

  rat lung tissue were subjected to SDS PAGE followed by western blot with 68123-1-Ig (PSMA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 68123-1-Ig

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 68123-1-Ig (PSMA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 68123-1-Ig

  WB result of PSMA8 antibody (68123-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA8 transfected HeLa cells.

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  IHC staining of human lung cancer using 68123-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 68123-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 68123-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 68123-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 68123-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 68123-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 68123-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 68123-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSMA8 antibody (68123-1-Ig, Clone: 2G2G3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, A549-Zellen, HeLa-Zellen, HuH-7-Zellen, Mauslungengewebe, NCI-H1299-Zellen, Hausschwein-Lungengewebe, Rattenlungengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe, Mauslebergewebe, Rattenlebergewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

68123-1-Ig bindet in WB, IHC, IF/ICC, ELISA PSMA8 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PSMA8 fusion protein Ag5138
• Vollständiger Name: proteasome (prosome, macropain) subunit, alpha type, 8
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC042820
• Gene symbol: PSMA8
• Gene ID (NCBI): 143471
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

1

Publikationen

Species	Application	Title
human	WB	Mol Cancer; Therapy-induced senescence is a transient drug resistance mechanism in breast cancer; Authors - Eszter Bajtai