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PSMB1 Monoklonaler Antikörper

PSMB1 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C7C5

Kat-Nr. : CL488-67847

Synonyme

FLJ25321, HC5, KIAA1838, Macropain subunit C5, PMSB1, Proteasome component C5, Proteasome gamma chain, Proteasome subunit beta type 1, PSC5, PSMB1

Galerie der Validierungsdaten

Filter:
  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated PSMB1 Monoclonal ant (CL488-67847)

    FC experiment of HeLa using CL488-67847

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PSMB1 (CL488-67847, Clone:1C7C5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67847 bindet in FC (Intra) PSMB1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen PSMB1 fusion protein Ag30791
Vollständiger Name proteasome (prosome, macropain) subunit, beta type, 1
Berechnetes Molekulargewicht 241 aa, 26 kDa
Beobachtetes Molekulargewicht25 kDa
GenBank-ZugangsnummerBC020807
Gene symbol PSMB1
Gene ID (NCBI) 5689
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMB1(Proteasome subunit beta type-1) is also named as PSC5 and belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The gene encodes a 241 amino acid protein with a 28 amino acid propeptide and two glycosylation sites.

Protokolle

Produktspezifische Protokolle
FC protocol for CL Plus 488 PSMB1 antibody CL488-67847Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen