PSMD10/Gankyrin Polyklonaler Antikörper

PSMD10/Gankyrin Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 12342-2-AP

Synonyme

PSMD10, 26S proteasome non-ATPase regulatory subunit 10, Gankyrin, p28, p28(GANK)

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  WB analysis using 12342-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12342-2-AP (PSMD10/Gankyrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human urothelial carcinoma using 12342-2-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12342-2-AP (PSMD10/Gankyrin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 12342-2-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12342-2-AP (PSMD10/Gankyrin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Urothelkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below

Produktinformation

12342-2-AP bindet in WB, IHC, ELISA PSMD10/Gankyrin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMD10/Gankyrin fusion protein Ag2994
• Vollständiger Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 10
• Berechnetes Molekulargewicht: 226 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24-28 kDa
• GenBank-Zugangsnummer: BC011960
• Gene symbol: PSMD10
• Gene ID (NCBI): 5716
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMD10, also known as Gankyrin, is a subunit of the 19S regulatory particle of the proteasome and plays a crucial role in protein degradation. It is a small, 25 kDa cytoplasmic-nuclear shuttling protein, highly conserved throughout evolution (~40% identity to yeast Nas6p). PSMD10 is involved in the regulation of cell cycle progression and apoptosis by interacting with key proteins such as p53 and Rb. PSMD10 has been identified as an oncoprotein, promoting tumor cell proliferation and survival in various cancers (PMID: 16581249, PMID: 31576540).

Publikationen

Species	Application	Title
mouse	WB	J Biomed Sci; Znf179 E3 ligase-mediated TDP-43 polyubiquitination is involved in TDP-43- ubiquitinated inclusions (UBI) (+)-related neurodegenerative pathology.; Authors - Yi-Chao Lee
human	WB	Appl Biochem Biotechnol; miR-188-5p and Host MALAT1 Regulate RBE Cell Migration, Invasion, and Apoptosis via Up-regulating PSMD10 in Cholangiocarcinoma; Authors - Zhaoqiang Yin
human	WB	Research (Wash D C); SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage; Authors - Fengli Wang
mouse,human	WB	Elife; A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression; Authors - Chaojun Yan
human	WB	Nat Commun; RNF167 mediates atypical ubiquitylation and degradation of RLRs via two distinct proteolytic pathways; Authors - Miao He ; KD Validated