PSMD13 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PSMD13 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 15261-1-AP

Synonyme

S11, Rpn9, p40.5, HSPC027, 26S proteasome non-ATPase regulatory subunit 13

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  WB analysis of HEK-293 using 15261-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15261-1-AP (PSMD13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293T using 15261-1-AP

  IP result of anti-PSMD13 (IP:15261-1-AP, 4ug; Detection:15261-1-AP 1:1000) with HEK-293T cells lysate 1200 ug.

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  IHC staining of human kidney using 15261-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15261-1-AP (PSMD13 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 15261-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15261-1-AP (PSMD13 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 15261-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15261-1-AP (PSMD13 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15261-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15261-1-AP (PSMD13 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15261-1-AP

  Immunofluorescent analysis of Hela cells, using PSMD13 antibody 15261-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Y79-Zellen
• Erfolgreiche IP: HEK-293T-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below

Produktinformation

15261-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PSMD13 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMD13 fusion protein Ag7257
• Vollständiger Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 13
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC001100
• Gene symbol: PSMD13
• Gene ID (NCBI): 5719
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	J Cell Biol; Proteasome gene expression is controlled by coordinated functions of multiple transcription factors; Authors - Jennifer E Gilda
mouse	WB	J Neurosci; Pharmacological Amelioration of Cone Survival and Vision in a Mouse Model for Leber Congenital Amaurosis.; Authors - Songhua Li ; KD Validated
mouse	WB,IHC	J Biol Chem; Rescue of enzymatic function for disease-associated RPE65s containing various missense mutations in non-active sites.; Authors - Songhua Li
	WB	J Biochem; Temperature sensitive retinoid isomerase activity of RPE65 mutants associated with Leber congenital amaurosis.; Authors - Songhua Li
mouse	WB	bioRxiv; Proteasome gene expression is controlled by the coordinated functions of multiple transcription factors; Authors - Jennifer E Gilda
human	WB,IHC	Mamm Genome; Comprehensive analysis reveals the prognostic and immunological role of PSMD13 in hepatocellular carcinoma; Authors - Yun Li