PSMD13 Monoklonaler Antikörper

PSMD13 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, Huhn, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2G10G6

Kat-Nr. : 67807-1-Ig

Synonyme

HSPC027, p40.5, PSMD13, Rpn9, S11

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Galerie der Validierungsdaten

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  WB analysis using 67807-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67807-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 67807-1-Ig

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 67807-1-Ig

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 67807-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67807-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 67807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67807-1-Ig (PSMD13 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 67807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67807-1-Ig (PSMD13 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67807-1-Ig (PSMD13 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67807-1-Ig (PSMD13 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67807-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSMD13 antibody (67807-1-Ig, Clone: 2G10G6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HuH-7-Zellen, Jurkat-Zellen, K-562-Zellen, Kaninchenlebergewebe, L02-Zellen, Mauslebergewebe, NIH/3T3-Zellen, Rattenlebergewebe, SMMC-7721-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67807-1-Ig bindet in WB, IHC, IF, ELISA PSMD13 und zeigt Reaktivität mit Hausschwein, Huhn, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, Huhn, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PSMD13 fusion protein Ag7857
• Vollständiger Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 13
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC001100
• Gene symbol: PSMD13
• Gene ID (NCBI): 5719
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.